Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma

Thomas W. Burke, Stanimir Vuk-Pavlović

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Human non-autocrine neuroblastoma cells SK-N-SH and LF require serum for proliferation in vitro. We wished to determine the role of serum-borne insulin-like growth factor I (IGF-I) as mitogen for these cells. Introduction of the monoclonal antibody α-IR3 against human IGF-I receptor reduced proliferation in the presence of fetal bovine serum (FBS). IGF-I (5 n M) was as effective as FBS (10%) in stimulating proliferation. Porcine insulin mimicked the effects of IGF-I, but at a 1000-fold higher concentration. The antibody α-IR3 reduced growth stimulated by IGF-I more effectively than growth stimulated by insulin. Thus, proliferation of human non-autocrine neuroblastoma cells can be effectively manipulated by exogenous IGF-I.

Original languageEnglish (US)
Pages (from-to)391-394
Number of pages4
JournalIn Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology
Volume29
Issue number5
DOIs
StatePublished - May 1993

Keywords

  • fetal bovine serum
  • insulin
  • insulin-like growth factor I
  • neuroblastoma
  • α-IR antibody

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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