Insulin increases ventilation during euglycemia in humans

Thales C. Barbosa, Jasdeep Kaur, Seth W. Holwerda, Colin N. Young, Timothy B Curry, John P. Thyfault, Michael Joseph Joyner, Jacqueline K. Limberg, Paul J. Fadel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Evidence from animal studies indicates that hyperinsulinemia, without changes in glucose, increases ventilation via a carotid body-mediated mechanism. However, whether insulin elevates ventilation in humans independently of changes in glucose remains unclear. Therefore, we tested the hypothesis that insulin increases ventilation in humans during a hyperinsulinemic-euglycemic clamp in which insulin was elevated to postprandial concentrations while glucose was maintained at fasting concentrations. First, in 16 healthy young men (protocol 1), we retrospectively analyzed respiration rate and estimated tidal volume from a pneumobelt to calculate minute ventilation during a hyperinsulinemic-euglycemic clamp. In addition, for a direct assessment of minute ventilation during a hyperinsulinemic-euglycemic clamp, we retrospectively analyzed breath-by-breath respiration rate and tidal volume from inspired/expired gasses in an additional 23 healthy young subjects (protocol 2). Clamp infusion elevated minute ventilation from baseline in both protocols (protocol 1: =11.9-4.6% baseline, P < 0.001; protocol 2: =9.5-3.8% baseline, P < 0.020). In protocol 1, peak changes in both respiration rate (=13.9-3.0% baseline, P < 0.001) and estimated tidal volume (=16.9-4.1% baseline, P < 0.001) were higher than baseline during the clamp. In protocol 2, tidal volume primarily increased during the clamp (=9.7-3.7% baseline, P < 0.016), as respiration rate did not change significantly (=0.2-1.8% baseline, P < 0.889). Collectively, we demonstrate for the first time in humans that elevated plasma insulin increases minute ventilation independent of changes in glucose.

Original languageEnglish (US)
Pages (from-to)R84-R89,
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume315
Issue number1
DOIs
StatePublished - Jul 2 2018

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Ventilation
Insulin
Tidal Volume
Respiratory Rate
Glucose Clamp Technique
Glucose
Carotid Body
Hyperinsulinism
Fasting
Healthy Volunteers

Keywords

  • Carotid body
  • Chemoreceptors
  • Respiration rate
  • Tidal volume

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Insulin increases ventilation during euglycemia in humans. / Barbosa, Thales C.; Kaur, Jasdeep; Holwerda, Seth W.; Young, Colin N.; Curry, Timothy B; Thyfault, John P.; Joyner, Michael Joseph; Limberg, Jacqueline K.; Fadel, Paul J.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 315, No. 1, 02.07.2018, p. R84-R89,.

Research output: Contribution to journalArticle

Barbosa, Thales C. ; Kaur, Jasdeep ; Holwerda, Seth W. ; Young, Colin N. ; Curry, Timothy B ; Thyfault, John P. ; Joyner, Michael Joseph ; Limberg, Jacqueline K. ; Fadel, Paul J. / Insulin increases ventilation during euglycemia in humans. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2018 ; Vol. 315, No. 1. pp. R84-R89,.
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AU - Kaur, Jasdeep

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AU - Curry, Timothy B

AU - Thyfault, John P.

AU - Joyner, Michael Joseph

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AB - Evidence from animal studies indicates that hyperinsulinemia, without changes in glucose, increases ventilation via a carotid body-mediated mechanism. However, whether insulin elevates ventilation in humans independently of changes in glucose remains unclear. Therefore, we tested the hypothesis that insulin increases ventilation in humans during a hyperinsulinemic-euglycemic clamp in which insulin was elevated to postprandial concentrations while glucose was maintained at fasting concentrations. First, in 16 healthy young men (protocol 1), we retrospectively analyzed respiration rate and estimated tidal volume from a pneumobelt to calculate minute ventilation during a hyperinsulinemic-euglycemic clamp. In addition, for a direct assessment of minute ventilation during a hyperinsulinemic-euglycemic clamp, we retrospectively analyzed breath-by-breath respiration rate and tidal volume from inspired/expired gasses in an additional 23 healthy young subjects (protocol 2). Clamp infusion elevated minute ventilation from baseline in both protocols (protocol 1: =11.9-4.6% baseline, P < 0.001; protocol 2: =9.5-3.8% baseline, P < 0.020). In protocol 1, peak changes in both respiration rate (=13.9-3.0% baseline, P < 0.001) and estimated tidal volume (=16.9-4.1% baseline, P < 0.001) were higher than baseline during the clamp. In protocol 2, tidal volume primarily increased during the clamp (=9.7-3.7% baseline, P < 0.016), as respiration rate did not change significantly (=0.2-1.8% baseline, P < 0.889). Collectively, we demonstrate for the first time in humans that elevated plasma insulin increases minute ventilation independent of changes in glucose.

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KW - Respiration rate

KW - Tidal volume

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