Insights into coronary collateral formation from a novel porcine semiacute infarction model

Florian Krackhardt, Jonathan M. Harnoss, Matthias W. Waliszewski, Zully Ritter, Susanne Granzow, Dieter Felsenberg, Konrad Neumann, Lilach O Lerman, Philipp Hillmeister, Rolf Gebker, Ingo Paetsch, Fabian Riediger, Peter Bramlage, Ivo R. Buschmann

Research output: Contribution to journalArticle

Abstract

Background For patients with severe ischemic heart disease, complete revascularization by a percutaneous coronary intervention or coronary artery bypass grafting is often not achieved and may still cause residual angina. In case of progressive coronary artery occlusions, therapeutic arteriogenesis constitutes a promising strategy for increasing blood supply to the ischemic myocardium. Whether the formation of collaterals in the hypofused myocardium is angiogenetic in nature or based on preformed coronary artery anastomoses remains debatable. The objectives of this research were (i) the development of an appropriate research methodology to study a humanoid animal semiacute infarction model with low mortality and (ii) to answer the question of whether collateral revascularization follows a pre-existing 'blueprint'. Materials and methods A porcine model was chosen in which a step-wise vessel occlusion was performed by implantation of a copper stent into the distal left anterior descending artery. Vessel occlusion and collateral development were confirmed in vivo every 14 days up to day 56 by repeated coronary angiography and myocardial perfusion measurement using cardiac MRI. After the completion of the in-vivo imaging studies, animals were euthanized and collateral growth was evaluated using microcomputer tomography. Results Our porcine model of semiacute noninvasive coronary artery occlusion confirmed the existence of preformed coronary anastomoses and the proliferation of functional vessels in hypoperfused myocardium. Repetitive intra-animal MRIs showed the functional impact of these growing collaterals. Conclusion The confirmation of preformed coronary anastomoses during the process of collateralization (natural bypasses) offers a preclinical avenue to carry out arteriogenetic pharmaceutical research in patients with ischemic heart disease.

Original languageEnglish (US)
Pages (from-to)127-137
Number of pages11
JournalCoronary Artery Disease
Volume29
Issue number2
DOIs
StatePublished - Jan 1 2018

Fingerprint

Infarction
Coronary Vessels
Myocardium
Swine
Coronary Occlusion
Myocardial Ischemia
Microcomputers
Percutaneous Coronary Intervention
Coronary Angiography
Coronary Artery Bypass
Stents
Copper
Research Design
Arteries
Perfusion
Tomography
Magnetic Resonance Imaging
Mortality
Growth
Research

Keywords

  • arteriogenesis
  • collateral growth
  • coronary artery
  • occlusion
  • porcine model

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Krackhardt, F., Harnoss, J. M., Waliszewski, M. W., Ritter, Z., Granzow, S., Felsenberg, D., ... Buschmann, I. R. (2018). Insights into coronary collateral formation from a novel porcine semiacute infarction model. Coronary Artery Disease, 29(2), 127-137. https://doi.org/10.1097/MCA.0000000000000569

Insights into coronary collateral formation from a novel porcine semiacute infarction model. / Krackhardt, Florian; Harnoss, Jonathan M.; Waliszewski, Matthias W.; Ritter, Zully; Granzow, Susanne; Felsenberg, Dieter; Neumann, Konrad; Lerman, Lilach O; Hillmeister, Philipp; Gebker, Rolf; Paetsch, Ingo; Riediger, Fabian; Bramlage, Peter; Buschmann, Ivo R.

In: Coronary Artery Disease, Vol. 29, No. 2, 01.01.2018, p. 127-137.

Research output: Contribution to journalArticle

Krackhardt, F, Harnoss, JM, Waliszewski, MW, Ritter, Z, Granzow, S, Felsenberg, D, Neumann, K, Lerman, LO, Hillmeister, P, Gebker, R, Paetsch, I, Riediger, F, Bramlage, P & Buschmann, IR 2018, 'Insights into coronary collateral formation from a novel porcine semiacute infarction model', Coronary Artery Disease, vol. 29, no. 2, pp. 127-137. https://doi.org/10.1097/MCA.0000000000000569
Krackhardt F, Harnoss JM, Waliszewski MW, Ritter Z, Granzow S, Felsenberg D et al. Insights into coronary collateral formation from a novel porcine semiacute infarction model. Coronary Artery Disease. 2018 Jan 1;29(2):127-137. https://doi.org/10.1097/MCA.0000000000000569
Krackhardt, Florian ; Harnoss, Jonathan M. ; Waliszewski, Matthias W. ; Ritter, Zully ; Granzow, Susanne ; Felsenberg, Dieter ; Neumann, Konrad ; Lerman, Lilach O ; Hillmeister, Philipp ; Gebker, Rolf ; Paetsch, Ingo ; Riediger, Fabian ; Bramlage, Peter ; Buschmann, Ivo R. / Insights into coronary collateral formation from a novel porcine semiacute infarction model. In: Coronary Artery Disease. 2018 ; Vol. 29, No. 2. pp. 127-137.
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abstract = "Background For patients with severe ischemic heart disease, complete revascularization by a percutaneous coronary intervention or coronary artery bypass grafting is often not achieved and may still cause residual angina. In case of progressive coronary artery occlusions, therapeutic arteriogenesis constitutes a promising strategy for increasing blood supply to the ischemic myocardium. Whether the formation of collaterals in the hypofused myocardium is angiogenetic in nature or based on preformed coronary artery anastomoses remains debatable. The objectives of this research were (i) the development of an appropriate research methodology to study a humanoid animal semiacute infarction model with low mortality and (ii) to answer the question of whether collateral revascularization follows a pre-existing 'blueprint'. Materials and methods A porcine model was chosen in which a step-wise vessel occlusion was performed by implantation of a copper stent into the distal left anterior descending artery. Vessel occlusion and collateral development were confirmed in vivo every 14 days up to day 56 by repeated coronary angiography and myocardial perfusion measurement using cardiac MRI. After the completion of the in-vivo imaging studies, animals were euthanized and collateral growth was evaluated using microcomputer tomography. Results Our porcine model of semiacute noninvasive coronary artery occlusion confirmed the existence of preformed coronary anastomoses and the proliferation of functional vessels in hypoperfused myocardium. Repetitive intra-animal MRIs showed the functional impact of these growing collaterals. Conclusion The confirmation of preformed coronary anastomoses during the process of collateralization (natural bypasses) offers a preclinical avenue to carry out arteriogenetic pharmaceutical research in patients with ischemic heart disease.",
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AU - Harnoss, Jonathan M.

AU - Waliszewski, Matthias W.

AU - Ritter, Zully

AU - Granzow, Susanne

AU - Felsenberg, Dieter

AU - Neumann, Konrad

AU - Lerman, Lilach O

AU - Hillmeister, Philipp

AU - Gebker, Rolf

AU - Paetsch, Ingo

AU - Riediger, Fabian

AU - Bramlage, Peter

AU - Buschmann, Ivo R.

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N2 - Background For patients with severe ischemic heart disease, complete revascularization by a percutaneous coronary intervention or coronary artery bypass grafting is often not achieved and may still cause residual angina. In case of progressive coronary artery occlusions, therapeutic arteriogenesis constitutes a promising strategy for increasing blood supply to the ischemic myocardium. Whether the formation of collaterals in the hypofused myocardium is angiogenetic in nature or based on preformed coronary artery anastomoses remains debatable. The objectives of this research were (i) the development of an appropriate research methodology to study a humanoid animal semiacute infarction model with low mortality and (ii) to answer the question of whether collateral revascularization follows a pre-existing 'blueprint'. Materials and methods A porcine model was chosen in which a step-wise vessel occlusion was performed by implantation of a copper stent into the distal left anterior descending artery. Vessel occlusion and collateral development were confirmed in vivo every 14 days up to day 56 by repeated coronary angiography and myocardial perfusion measurement using cardiac MRI. After the completion of the in-vivo imaging studies, animals were euthanized and collateral growth was evaluated using microcomputer tomography. Results Our porcine model of semiacute noninvasive coronary artery occlusion confirmed the existence of preformed coronary anastomoses and the proliferation of functional vessels in hypoperfused myocardium. Repetitive intra-animal MRIs showed the functional impact of these growing collaterals. Conclusion The confirmation of preformed coronary anastomoses during the process of collateralization (natural bypasses) offers a preclinical avenue to carry out arteriogenetic pharmaceutical research in patients with ischemic heart disease.

AB - Background For patients with severe ischemic heart disease, complete revascularization by a percutaneous coronary intervention or coronary artery bypass grafting is often not achieved and may still cause residual angina. In case of progressive coronary artery occlusions, therapeutic arteriogenesis constitutes a promising strategy for increasing blood supply to the ischemic myocardium. Whether the formation of collaterals in the hypofused myocardium is angiogenetic in nature or based on preformed coronary artery anastomoses remains debatable. The objectives of this research were (i) the development of an appropriate research methodology to study a humanoid animal semiacute infarction model with low mortality and (ii) to answer the question of whether collateral revascularization follows a pre-existing 'blueprint'. Materials and methods A porcine model was chosen in which a step-wise vessel occlusion was performed by implantation of a copper stent into the distal left anterior descending artery. Vessel occlusion and collateral development were confirmed in vivo every 14 days up to day 56 by repeated coronary angiography and myocardial perfusion measurement using cardiac MRI. After the completion of the in-vivo imaging studies, animals were euthanized and collateral growth was evaluated using microcomputer tomography. Results Our porcine model of semiacute noninvasive coronary artery occlusion confirmed the existence of preformed coronary anastomoses and the proliferation of functional vessels in hypoperfused myocardium. Repetitive intra-animal MRIs showed the functional impact of these growing collaterals. Conclusion The confirmation of preformed coronary anastomoses during the process of collateralization (natural bypasses) offers a preclinical avenue to carry out arteriogenetic pharmaceutical research in patients with ischemic heart disease.

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KW - collateral growth

KW - coronary artery

KW - occlusion

KW - porcine model

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