Insertion of excised IgH switch sequences causes overexpression of cyclin D1 in a myeloma tumor cell

Ana Gabrea, P. Leif Bergsagel, Marta Chesi, Yaping Shou, W. Michael Kuehl

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

Oncogenes are often dysregulated in B cell tumors as a result of a reciprocal translocation involving an immunoglobulin locus. The translocations are caused by errors in two developmentally regulated DNA recombination processes: V(D)J and IgH switch recombination. Both processes share the property of joining discontinuous sequences from one chromosome and releasing intervening sequences as circles that are lost from progeny cells. Here we show that these intervening sequences may instead insert in the genome and that during productive IgH mu-epsilon switch recombination in U266 myeloma tumor cells, a portion of the excised IgH switch intervening sequences containing the 3' α-1 enhancer has inserted on chromosome 11q13, resulting in overexpression of the adjacent cyclin D1 oncogene.

Original languageEnglish (US)
Pages (from-to)119-123
Number of pages5
JournalMolecular Cell
Volume3
Issue number1
DOIs
StatePublished - Jan 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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