Inorganic phosphate nanorods are a novel fluorescent label in cell biology

Chitta Ranjan Patra, Resham Bhattacharya, Sujata Patra, Sujit Basu, Priyabrata Mukherjee, Debabrata Mukhopadhyay

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

We report the first use of inorganic fluorescent lanthanide (europium and terbium) ortho phosphate [LnPO4·H2O, Ln = Eu and Tb] nanorods as a novel fluorescent label in cell biology. These nanorods, synthesized by the microwave technique, retain their fluorescent properties after internalization into human umbilical vein endothelial cells (HUVEC), 786-O cells, or renal carcinoma cells (RCC). The cellular internalization of these nanorods and their fluorescence properties were characterized by fluorescence spectroscopy (FS), differential interference contrast (DIC) microscopy, confocal microscopy, and transmission electron microscopy (TEM). At concentrations up to 50 μg/ ml, the use of [3H]-thymidine incorporation assays, apoptosis assays (TUNEL), and trypan blue exclusion illustrated the non-toxic nature of these nanorods, a major advantage over traditional organic dyes.

Original languageEnglish (US)
Article number11
JournalJournal of Nanobiotechnology
Volume4
DOIs
StatePublished - Oct 30 2006

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Pharmaceutical Science

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