Innate immune function after breast, lung, and colorectal cancer surgery

Maria F. Ramirez, Di Ai, Maria Bauer, Jean Nicolas Vauthey, Vijaya Gottumukkala, Spencer Kee, Daliah Shon, Mark Truty, Henry M. Kuerer, Anrea Kurz, Mike Hernandez, Juan P. Cata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background The cytotoxic activity and count of natural killer (NK) cells appear to be reduced after surgery; however, it is unknown whether the magnitude of this immune suppression is similar among different types of oncological surgery. In this study, we compared the innate immune function of patients undergoing three different oncological surgeries. Methods We compared the number and function of NK cells obtained from patients who had undergone mastectomies (n = 17), thoracotomies (n = 21), or liver resections for cancer (n = 22). Cytotoxicity assays were performed to measure the function of NK cells. We also determined the plasma concentrations of interleukins (IL) 2 and 4, interferon-γ, granzyme B, perforin, soluble major histocompatibility complex class I-related chain A, and epinephrine, both before and 24 h after surgery. Differences in immunologic parameters were compared preoperatively and postoperatively and by type of surgery. P values <0.05 were considered statistically significant. Results The preoperative NK cell count differed statistically (P < 0.006) among all three types of surgeries; however, within surgery postoperative counts and changes compared with baseline did not. The postoperative function of NK cells was similar among types of surgeries, but was significantly reduced compared with preoperative levels (mastectomy P < 0.0001, thoracotomy P = 0.001, and liver resections P = 0.002). We observed a significant increase in the postoperative plasma concentrations of epinephrine, whereas the concentrations of major histocompatibility class I polypeptide-related sequence A and the IL-2 and/or IL-4 ratio remained unchanged before and after surgery. Conclusions The magnitude of innate immune suppression is similar among different oncological procedures. More studies are needed to better understand this complex phenomenon.

Original languageEnglish (US)
Pages (from-to)185-193
Number of pages9
JournalJournal of Surgical Research
Volume194
Issue number1
DOIs
StatePublished - Jan 1 2015

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Colorectal Surgery
Colorectal Neoplasms
Lung Neoplasms
Breast Neoplasms
Natural Killer Cells
Mastectomy
Thoracotomy
Interleukin-4
Epinephrine
Interleukin-2
Histocompatibility
Liver Neoplasms
Major Histocompatibility Complex
Interferons
Cell Count
Peptides
Liver

Keywords

  • Cancer surgery
  • Natural killer cells
  • Surgical stress

ASJC Scopus subject areas

  • Surgery

Cite this

Ramirez, M. F., Ai, D., Bauer, M., Vauthey, J. N., Gottumukkala, V., Kee, S., ... Cata, J. P. (2015). Innate immune function after breast, lung, and colorectal cancer surgery. Journal of Surgical Research, 194(1), 185-193. https://doi.org/10.1016/j.jss.2014.10.030

Innate immune function after breast, lung, and colorectal cancer surgery. / Ramirez, Maria F.; Ai, Di; Bauer, Maria; Vauthey, Jean Nicolas; Gottumukkala, Vijaya; Kee, Spencer; Shon, Daliah; Truty, Mark; Kuerer, Henry M.; Kurz, Anrea; Hernandez, Mike; Cata, Juan P.

In: Journal of Surgical Research, Vol. 194, No. 1, 01.01.2015, p. 185-193.

Research output: Contribution to journalArticle

Ramirez, MF, Ai, D, Bauer, M, Vauthey, JN, Gottumukkala, V, Kee, S, Shon, D, Truty, M, Kuerer, HM, Kurz, A, Hernandez, M & Cata, JP 2015, 'Innate immune function after breast, lung, and colorectal cancer surgery', Journal of Surgical Research, vol. 194, no. 1, pp. 185-193. https://doi.org/10.1016/j.jss.2014.10.030
Ramirez MF, Ai D, Bauer M, Vauthey JN, Gottumukkala V, Kee S et al. Innate immune function after breast, lung, and colorectal cancer surgery. Journal of Surgical Research. 2015 Jan 1;194(1):185-193. https://doi.org/10.1016/j.jss.2014.10.030
Ramirez, Maria F. ; Ai, Di ; Bauer, Maria ; Vauthey, Jean Nicolas ; Gottumukkala, Vijaya ; Kee, Spencer ; Shon, Daliah ; Truty, Mark ; Kuerer, Henry M. ; Kurz, Anrea ; Hernandez, Mike ; Cata, Juan P. / Innate immune function after breast, lung, and colorectal cancer surgery. In: Journal of Surgical Research. 2015 ; Vol. 194, No. 1. pp. 185-193.
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abstract = "Background The cytotoxic activity and count of natural killer (NK) cells appear to be reduced after surgery; however, it is unknown whether the magnitude of this immune suppression is similar among different types of oncological surgery. In this study, we compared the innate immune function of patients undergoing three different oncological surgeries. Methods We compared the number and function of NK cells obtained from patients who had undergone mastectomies (n = 17), thoracotomies (n = 21), or liver resections for cancer (n = 22). Cytotoxicity assays were performed to measure the function of NK cells. We also determined the plasma concentrations of interleukins (IL) 2 and 4, interferon-γ, granzyme B, perforin, soluble major histocompatibility complex class I-related chain A, and epinephrine, both before and 24 h after surgery. Differences in immunologic parameters were compared preoperatively and postoperatively and by type of surgery. P values <0.05 were considered statistically significant. Results The preoperative NK cell count differed statistically (P < 0.006) among all three types of surgeries; however, within surgery postoperative counts and changes compared with baseline did not. The postoperative function of NK cells was similar among types of surgeries, but was significantly reduced compared with preoperative levels (mastectomy P < 0.0001, thoracotomy P = 0.001, and liver resections P = 0.002). We observed a significant increase in the postoperative plasma concentrations of epinephrine, whereas the concentrations of major histocompatibility class I polypeptide-related sequence A and the IL-2 and/or IL-4 ratio remained unchanged before and after surgery. Conclusions The magnitude of innate immune suppression is similar among different oncological procedures. More studies are needed to better understand this complex phenomenon.",
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AU - Kee, Spencer

AU - Shon, Daliah

AU - Truty, Mark

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N2 - Background The cytotoxic activity and count of natural killer (NK) cells appear to be reduced after surgery; however, it is unknown whether the magnitude of this immune suppression is similar among different types of oncological surgery. In this study, we compared the innate immune function of patients undergoing three different oncological surgeries. Methods We compared the number and function of NK cells obtained from patients who had undergone mastectomies (n = 17), thoracotomies (n = 21), or liver resections for cancer (n = 22). Cytotoxicity assays were performed to measure the function of NK cells. We also determined the plasma concentrations of interleukins (IL) 2 and 4, interferon-γ, granzyme B, perforin, soluble major histocompatibility complex class I-related chain A, and epinephrine, both before and 24 h after surgery. Differences in immunologic parameters were compared preoperatively and postoperatively and by type of surgery. P values <0.05 were considered statistically significant. Results The preoperative NK cell count differed statistically (P < 0.006) among all three types of surgeries; however, within surgery postoperative counts and changes compared with baseline did not. The postoperative function of NK cells was similar among types of surgeries, but was significantly reduced compared with preoperative levels (mastectomy P < 0.0001, thoracotomy P = 0.001, and liver resections P = 0.002). We observed a significant increase in the postoperative plasma concentrations of epinephrine, whereas the concentrations of major histocompatibility class I polypeptide-related sequence A and the IL-2 and/or IL-4 ratio remained unchanged before and after surgery. Conclusions The magnitude of innate immune suppression is similar among different oncological procedures. More studies are needed to better understand this complex phenomenon.

AB - Background The cytotoxic activity and count of natural killer (NK) cells appear to be reduced after surgery; however, it is unknown whether the magnitude of this immune suppression is similar among different types of oncological surgery. In this study, we compared the innate immune function of patients undergoing three different oncological surgeries. Methods We compared the number and function of NK cells obtained from patients who had undergone mastectomies (n = 17), thoracotomies (n = 21), or liver resections for cancer (n = 22). Cytotoxicity assays were performed to measure the function of NK cells. We also determined the plasma concentrations of interleukins (IL) 2 and 4, interferon-γ, granzyme B, perforin, soluble major histocompatibility complex class I-related chain A, and epinephrine, both before and 24 h after surgery. Differences in immunologic parameters were compared preoperatively and postoperatively and by type of surgery. P values <0.05 were considered statistically significant. Results The preoperative NK cell count differed statistically (P < 0.006) among all three types of surgeries; however, within surgery postoperative counts and changes compared with baseline did not. The postoperative function of NK cells was similar among types of surgeries, but was significantly reduced compared with preoperative levels (mastectomy P < 0.0001, thoracotomy P = 0.001, and liver resections P = 0.002). We observed a significant increase in the postoperative plasma concentrations of epinephrine, whereas the concentrations of major histocompatibility class I polypeptide-related sequence A and the IL-2 and/or IL-4 ratio remained unchanged before and after surgery. Conclusions The magnitude of innate immune suppression is similar among different oncological procedures. More studies are needed to better understand this complex phenomenon.

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