Inhibitory influence of IL-4 on human B cell responsiveness

Diane F Jelinek, P. E. Lipsky

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Abstract

The role of IL-4 in human B cell activation, proliferation, and differentiation was examined. rIL-2, but not rIL-4, was able to promote maximum proliferation and generation of Ig-secreting cells in cultures of highly purified B cells stimulated with Cowan I Staphylococcus aureus (SA). Addition of rIL-4 to rIL-2-supported cultures of SA-stimulated peripheral blood, spleen, or lymph node B cells dramatically suppressed both proliferation and differentiation. Results from experiments in which rIL-4 was added to culture at progressively later times indicated a requirement for rIL-4 to be present during the first 2 days of a 5-day incubation to cause inhibition of responsiveness. When a two-stage culture system was utilized, rIL-4 was found to support proliferation or differentiation of B cells initially activated with SA for 2 days only minimally, How ever, rIL-4 did not inhibit responses of SA preactivated B cells supported by IL-2. The presence of rIL-4 during the initial 48-h activation of B cells with SA and rIL-2 resulted in a profound inhibition of the ability of the activated B cells to respond subsequently to rIL-2 or lymphokine-rich T cell supernatants. A similar 48-h incubation with rIL-4 alone without SA had no effect on subsequent B cell responsiveness. The presence of rIFN-γ during B cell activation decreased the inhibitory effect of IL-4. Other cytokines including IFN-α, IL-1, and commercially available low m.w. B cell growth factor also diminished the inhibitory effect of IL-4. These results indicate that IL-4 inhibits the capacity of human B cells to be activated maximally by SA and rIL-2 and therefore suggest a new immunomodulatory role for this cytokine.

Original languageEnglish (US)
Pages (from-to)164-173
Number of pages10
JournalJournal of Immunology
Volume141
Issue number1
StatePublished - 1988
Externally publishedYes

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Interleukin-4
B-Lymphocytes
Staphylococcus aureus
Cytokines
Lymphokines
Interleukin-1
Interleukin-2
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Spleen
Cell Culture Techniques
Lymph Nodes
Cell Proliferation
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Inhibitory influence of IL-4 on human B cell responsiveness. / Jelinek, Diane F; Lipsky, P. E.

In: Journal of Immunology, Vol. 141, No. 1, 1988, p. 164-173.

Research output: Contribution to journalArticle

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