Inhibition of protein kinase A phase delays the mammalian circadian clock

Jennifer M. Lee; Kathryn M. Schak; Mary E. Harrington

doi:10.1016/S0006-8993(99)01600-5

Inhibition of protein kinase A phase delays the mammalian circadian clock

Jennifer M. Lee, Kathryn M. Schak, Mary E. Harrington

Psychiatry and Psychology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The suprachiasmatic nuclei (SCN) contain the mammalian circadian clock whose rhythm of firing rate can be recorded in vitro for several days. Application of a protein kinase A (PKA) inhibitor onto the SCN at Zeitgeber time (ZT) 10 on the first day in vitro phase delayed the rhythm of firing rate expressed by SCN neurons on the subsequent day in vitro. Application of the inhibitor (Rp-cAMPS) at other circadian phases did not phase shift the rhythm. These results suggest that during approximately 1 h in the late subjective day the presence and activity of PKA plays a role in setting the phase of the mammalian circadian clock.

Original language	English (US)
Pages (from-to)	350-353
Number of pages	4
Journal	Brain Research
Volume	835
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(99)01600-5
State	Published - Jul 24 1999

Keywords

Adenylate cyclase
Circadian
Cyclic AMP
Protein kinase A
Rhythm
Suprachiasmatic

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(99)01600-5

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title = "Inhibition of protein kinase A phase delays the mammalian circadian clock",

abstract = "The suprachiasmatic nuclei (SCN) contain the mammalian circadian clock whose rhythm of firing rate can be recorded in vitro for several days. Application of a protein kinase A (PKA) inhibitor onto the SCN at Zeitgeber time (ZT) 10 on the first day in vitro phase delayed the rhythm of firing rate expressed by SCN neurons on the subsequent day in vitro. Application of the inhibitor (Rp-cAMPS) at other circadian phases did not phase shift the rhythm. These results suggest that during approximately 1 h in the late subjective day the presence and activity of PKA plays a role in setting the phase of the mammalian circadian clock.",

keywords = "Adenylate cyclase, Circadian, Cyclic AMP, Protein kinase A, Rhythm, Suprachiasmatic",

author = "Lee, {Jennifer M.} and Schak, {Kathryn M.} and Harrington, {Mary E.}",

note = "Funding Information: This study was supported by NIH grant NS26496 (M.E.H.). The technical assistance of Jennifer L. Meyer is gratefully acknowledged. We would like to thank Gabriela A. Ferreyra and Chiaki Fukuhara for helpful discussions.",

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T1 - Inhibition of protein kinase A phase delays the mammalian circadian clock

AU - Lee, Jennifer M.

AU - Schak, Kathryn M.

AU - Harrington, Mary E.

N1 - Funding Information: This study was supported by NIH grant NS26496 (M.E.H.). The technical assistance of Jennifer L. Meyer is gratefully acknowledged. We would like to thank Gabriela A. Ferreyra and Chiaki Fukuhara for helpful discussions.

PY - 1999/7/24

Y1 - 1999/7/24

N2 - The suprachiasmatic nuclei (SCN) contain the mammalian circadian clock whose rhythm of firing rate can be recorded in vitro for several days. Application of a protein kinase A (PKA) inhibitor onto the SCN at Zeitgeber time (ZT) 10 on the first day in vitro phase delayed the rhythm of firing rate expressed by SCN neurons on the subsequent day in vitro. Application of the inhibitor (Rp-cAMPS) at other circadian phases did not phase shift the rhythm. These results suggest that during approximately 1 h in the late subjective day the presence and activity of PKA plays a role in setting the phase of the mammalian circadian clock.

AB - The suprachiasmatic nuclei (SCN) contain the mammalian circadian clock whose rhythm of firing rate can be recorded in vitro for several days. Application of a protein kinase A (PKA) inhibitor onto the SCN at Zeitgeber time (ZT) 10 on the first day in vitro phase delayed the rhythm of firing rate expressed by SCN neurons on the subsequent day in vitro. Application of the inhibitor (Rp-cAMPS) at other circadian phases did not phase shift the rhythm. These results suggest that during approximately 1 h in the late subjective day the presence and activity of PKA plays a role in setting the phase of the mammalian circadian clock.

KW - Adenylate cyclase

KW - Circadian

KW - Cyclic AMP

KW - Protein kinase A

KW - Rhythm

KW - Suprachiasmatic

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Inhibition of protein kinase A phase delays the mammalian circadian clock

Abstract

Keywords

ASJC Scopus subject areas

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