Inhibition of protein kinase A phase delays the mammalian circadian clock

Jennifer M. Lee, Kathryn M. Schak, Mary E. Harrington

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The suprachiasmatic nuclei (SCN) contain the mammalian circadian clock whose rhythm of firing rate can be recorded in vitro for several days. Application of a protein kinase A (PKA) inhibitor onto the SCN at Zeitgeber time (ZT) 10 on the first day in vitro phase delayed the rhythm of firing rate expressed by SCN neurons on the subsequent day in vitro. Application of the inhibitor (Rp-cAMPS) at other circadian phases did not phase shift the rhythm. These results suggest that during approximately 1 h in the late subjective day the presence and activity of PKA plays a role in setting the phase of the mammalian circadian clock.

Original languageEnglish (US)
Pages (from-to)350-353
Number of pages4
JournalBrain Research
Volume835
Issue number2
DOIs
StatePublished - Jul 24 1999

Keywords

  • Adenylate cyclase
  • Circadian
  • Cyclic AMP
  • Protein kinase A
  • Rhythm
  • Suprachiasmatic

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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