Inhibition of duck hepatitis B virus replication by hypericin

Gloria Moraleda, Tsung Teh Wu, Allison R. Jilbert, Carol E. Aldrich, Lynn D. Condreay, Steven H. Larsen, Joseph C. Tang, Joseph M. Colacino, William S. Mason

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Hypericin was found to be active against a member of the hepatitis B virus family, duck hepatitis B virus (DHBV). After a single 1 h incubation with hypericin, cells stably-transfected with a clone of DHBV stopped producing infectious virus for several days, though virus-like particles continued to be released into the culture medium. Characterization of these virions revealed a buoyant density characteristic of infectious virus preparations and lower than that of virus cores, suggesting that the particles were enveloped. Western blot analysis suggested, however, that the viral preS protein in surface antigen particles and, by inference, in virions, was present in covalently cross-linked aggregates. Evidence of a similar level of aggregation of the core subunit of virion nucleocapsids was not found, nor was there evidence of a similar high level of aggregation of cell-associated core and preS proteins. Hypericin was only slightly virucidal against DHBV and culture medium from treated cultures did not block initiation of infection when added to DHBV susceptible cultures prior to a challenge with infectious DHBV. Thus, the primary antiviral activity of hypericin against DHBV replication appears to be exerted at a late step in viral morphogenesis.

Original languageEnglish (US)
Pages (from-to)235-247
Number of pages13
JournalAntiviral Research
Volume20
Issue number3
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Duck Hepatitis B Viruses
Virus Replication
Virion
Viruses
Culture Media
Hepatovirus
Nucleocapsid
Cell Aggregation
Viral Proteins
Surface Antigens
Morphogenesis
Hepatitis B virus
Antiviral Agents
hypericin
Clone Cells
Western Blotting
Infection

Keywords

  • Duck Hepatitis B virus
  • Hepatocyte
  • Hypericin

ASJC Scopus subject areas

  • Virology
  • Pharmacology

Cite this

Moraleda, G., Wu, T. T., Jilbert, A. R., Aldrich, C. E., Condreay, L. D., Larsen, S. H., ... Mason, W. S. (1993). Inhibition of duck hepatitis B virus replication by hypericin. Antiviral Research, 20(3), 235-247. https://doi.org/10.1016/0166-3542(93)90023-C

Inhibition of duck hepatitis B virus replication by hypericin. / Moraleda, Gloria; Wu, Tsung Teh; Jilbert, Allison R.; Aldrich, Carol E.; Condreay, Lynn D.; Larsen, Steven H.; Tang, Joseph C.; Colacino, Joseph M.; Mason, William S.

In: Antiviral Research, Vol. 20, No. 3, 1993, p. 235-247.

Research output: Contribution to journalArticle

Moraleda, G, Wu, TT, Jilbert, AR, Aldrich, CE, Condreay, LD, Larsen, SH, Tang, JC, Colacino, JM & Mason, WS 1993, 'Inhibition of duck hepatitis B virus replication by hypericin', Antiviral Research, vol. 20, no. 3, pp. 235-247. https://doi.org/10.1016/0166-3542(93)90023-C
Moraleda G, Wu TT, Jilbert AR, Aldrich CE, Condreay LD, Larsen SH et al. Inhibition of duck hepatitis B virus replication by hypericin. Antiviral Research. 1993;20(3):235-247. https://doi.org/10.1016/0166-3542(93)90023-C
Moraleda, Gloria ; Wu, Tsung Teh ; Jilbert, Allison R. ; Aldrich, Carol E. ; Condreay, Lynn D. ; Larsen, Steven H. ; Tang, Joseph C. ; Colacino, Joseph M. ; Mason, William S. / Inhibition of duck hepatitis B virus replication by hypericin. In: Antiviral Research. 1993 ; Vol. 20, No. 3. pp. 235-247.
@article{a464e759dcfe440eb630dad7b56f4480,
title = "Inhibition of duck hepatitis B virus replication by hypericin",
abstract = "Hypericin was found to be active against a member of the hepatitis B virus family, duck hepatitis B virus (DHBV). After a single 1 h incubation with hypericin, cells stably-transfected with a clone of DHBV stopped producing infectious virus for several days, though virus-like particles continued to be released into the culture medium. Characterization of these virions revealed a buoyant density characteristic of infectious virus preparations and lower than that of virus cores, suggesting that the particles were enveloped. Western blot analysis suggested, however, that the viral preS protein in surface antigen particles and, by inference, in virions, was present in covalently cross-linked aggregates. Evidence of a similar level of aggregation of the core subunit of virion nucleocapsids was not found, nor was there evidence of a similar high level of aggregation of cell-associated core and preS proteins. Hypericin was only slightly virucidal against DHBV and culture medium from treated cultures did not block initiation of infection when added to DHBV susceptible cultures prior to a challenge with infectious DHBV. Thus, the primary antiviral activity of hypericin against DHBV replication appears to be exerted at a late step in viral morphogenesis.",
keywords = "Duck Hepatitis B virus, Hepatocyte, Hypericin",
author = "Gloria Moraleda and Wu, {Tsung Teh} and Jilbert, {Allison R.} and Aldrich, {Carol E.} and Condreay, {Lynn D.} and Larsen, {Steven H.} and Tang, {Joseph C.} and Colacino, {Joseph M.} and Mason, {William S.}",
year = "1993",
doi = "10.1016/0166-3542(93)90023-C",
language = "English (US)",
volume = "20",
pages = "235--247",
journal = "Antiviral Research",
issn = "0166-3542",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Inhibition of duck hepatitis B virus replication by hypericin

AU - Moraleda, Gloria

AU - Wu, Tsung Teh

AU - Jilbert, Allison R.

AU - Aldrich, Carol E.

AU - Condreay, Lynn D.

AU - Larsen, Steven H.

AU - Tang, Joseph C.

AU - Colacino, Joseph M.

AU - Mason, William S.

PY - 1993

Y1 - 1993

N2 - Hypericin was found to be active against a member of the hepatitis B virus family, duck hepatitis B virus (DHBV). After a single 1 h incubation with hypericin, cells stably-transfected with a clone of DHBV stopped producing infectious virus for several days, though virus-like particles continued to be released into the culture medium. Characterization of these virions revealed a buoyant density characteristic of infectious virus preparations and lower than that of virus cores, suggesting that the particles were enveloped. Western blot analysis suggested, however, that the viral preS protein in surface antigen particles and, by inference, in virions, was present in covalently cross-linked aggregates. Evidence of a similar level of aggregation of the core subunit of virion nucleocapsids was not found, nor was there evidence of a similar high level of aggregation of cell-associated core and preS proteins. Hypericin was only slightly virucidal against DHBV and culture medium from treated cultures did not block initiation of infection when added to DHBV susceptible cultures prior to a challenge with infectious DHBV. Thus, the primary antiviral activity of hypericin against DHBV replication appears to be exerted at a late step in viral morphogenesis.

AB - Hypericin was found to be active against a member of the hepatitis B virus family, duck hepatitis B virus (DHBV). After a single 1 h incubation with hypericin, cells stably-transfected with a clone of DHBV stopped producing infectious virus for several days, though virus-like particles continued to be released into the culture medium. Characterization of these virions revealed a buoyant density characteristic of infectious virus preparations and lower than that of virus cores, suggesting that the particles were enveloped. Western blot analysis suggested, however, that the viral preS protein in surface antigen particles and, by inference, in virions, was present in covalently cross-linked aggregates. Evidence of a similar level of aggregation of the core subunit of virion nucleocapsids was not found, nor was there evidence of a similar high level of aggregation of cell-associated core and preS proteins. Hypericin was only slightly virucidal against DHBV and culture medium from treated cultures did not block initiation of infection when added to DHBV susceptible cultures prior to a challenge with infectious DHBV. Thus, the primary antiviral activity of hypericin against DHBV replication appears to be exerted at a late step in viral morphogenesis.

KW - Duck Hepatitis B virus

KW - Hepatocyte

KW - Hypericin

UR - http://www.scopus.com/inward/record.url?scp=0027528904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027528904&partnerID=8YFLogxK

U2 - 10.1016/0166-3542(93)90023-C

DO - 10.1016/0166-3542(93)90023-C

M3 - Article

C2 - 8470884

AN - SCOPUS:0027528904

VL - 20

SP - 235

EP - 247

JO - Antiviral Research

JF - Antiviral Research

SN - 0166-3542

IS - 3

ER -