Inhibition of DNA binding proteins by oligonucleotide-directed triple helix formation

Louis J. Maher, Barbara Wold, Peter B. Dervan

Research output: Contribution to journalArticlepeer-review

412 Scopus citations

Abstract

Oligonucleotides that bind to duplex DNA in a sequence-specific manner by triple helix formation offer an approach to the experimental manipulation of sequence-specific protein binding. Micromolar concentrations of pyrimidine oligodeoxyribonucleotides are shown to block recognition of double helical DNA by prokaryotic modifying enzymes and a eukaryotic transcription factor at a homopurine target site. Inhibition is sequence-specific. Oligonucleotides containing 5-methylcytosine provide substantially more efficient inhibition than oligonucleotides containing cytosine. The results have implications for gene-specific repression by oligonucleotides or their analogs.

Original languageEnglish (US)
Pages (from-to)725-730
Number of pages6
JournalScience
Volume245
Issue number4919
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • General

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