Inhibition of cyclooxygenase-2 ameliorates the severity of pancreatitis and associated lung injury

Albert M. Song, Lakshmi Bhagat, Vijay Prem Singh, Gijs G D Van Acker, Michael L. Steer, Ashok K. Saluja

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Cyclooxygenase-2 (COX-2), a widely distributed enzyme, plays an important role in inflammation. We have studied the role of COX-2 in acute pancreatitis and pancreatitis-associated lung injury using both the pharmacological inhibition of COX-2 and genetic deletion of COX-2. Pancreatitis was induced in mice by 12 hourly injections of cerulein. The severity of pancreatitis was assessed by measuring serum amylase, pancreatic trypsin activity, intrapancreatic sequestration of neutrophils, and acinar cell necrosis. The severity of lung injury was evaluated by measuring lactate dehydrogenase levels in the bronchoalveolar lavage fluid and by quantitating neutrophil sequestration in the lung. In both the pharmacologically inhibited and genetically altered mice, the severity of pancreatitis and pancreatitis-associated lung injury was reduced compared with the noninhibited strains of COX-2-sufficient mice. This reduction in injury indicates that COX-2 plays an important proinflammatory role in pancreatitis and its associated lung injury. Our findings support the concept that COX-2 inhibitors may play a beneficial role in the prevention of acute pancreatitis or in the reduction of its severity.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume283
Issue number5 46-5
StatePublished - Nov 2002

Fingerprint

Lung Injury
Cyclooxygenase 2
Pancreatitis
Neutrophils
Ceruletide
Acinar Cells
Cyclooxygenase 2 Inhibitors
Bronchoalveolar Lavage Fluid
Amylases
L-Lactate Dehydrogenase
Trypsin
Necrosis
Pharmacology
Inflammation
Lung
Injections
Wounds and Injuries
Enzymes
Serum

Keywords

  • Cerulein
  • Heat shock protein 70
  • Inducible nitric oxide synthase
  • Myeloperoxidase
  • Neutrophil
  • Proinflammatory
  • Prostaglandin
  • Trypsin

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Inhibition of cyclooxygenase-2 ameliorates the severity of pancreatitis and associated lung injury. / Song, Albert M.; Bhagat, Lakshmi; Singh, Vijay Prem; Van Acker, Gijs G D; Steer, Michael L.; Saluja, Ashok K.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 283, No. 5 46-5, 11.2002.

Research output: Contribution to journalArticle

Song, Albert M. ; Bhagat, Lakshmi ; Singh, Vijay Prem ; Van Acker, Gijs G D ; Steer, Michael L. ; Saluja, Ashok K. / Inhibition of cyclooxygenase-2 ameliorates the severity of pancreatitis and associated lung injury. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 283, No. 5 46-5.
@article{45602ddc72c84ee484b4cc716038c5c5,
title = "Inhibition of cyclooxygenase-2 ameliorates the severity of pancreatitis and associated lung injury",
abstract = "Cyclooxygenase-2 (COX-2), a widely distributed enzyme, plays an important role in inflammation. We have studied the role of COX-2 in acute pancreatitis and pancreatitis-associated lung injury using both the pharmacological inhibition of COX-2 and genetic deletion of COX-2. Pancreatitis was induced in mice by 12 hourly injections of cerulein. The severity of pancreatitis was assessed by measuring serum amylase, pancreatic trypsin activity, intrapancreatic sequestration of neutrophils, and acinar cell necrosis. The severity of lung injury was evaluated by measuring lactate dehydrogenase levels in the bronchoalveolar lavage fluid and by quantitating neutrophil sequestration in the lung. In both the pharmacologically inhibited and genetically altered mice, the severity of pancreatitis and pancreatitis-associated lung injury was reduced compared with the noninhibited strains of COX-2-sufficient mice. This reduction in injury indicates that COX-2 plays an important proinflammatory role in pancreatitis and its associated lung injury. Our findings support the concept that COX-2 inhibitors may play a beneficial role in the prevention of acute pancreatitis or in the reduction of its severity.",
keywords = "Cerulein, Heat shock protein 70, Inducible nitric oxide synthase, Myeloperoxidase, Neutrophil, Proinflammatory, Prostaglandin, Trypsin",
author = "Song, {Albert M.} and Lakshmi Bhagat and Singh, {Vijay Prem} and {Van Acker}, {Gijs G D} and Steer, {Michael L.} and Saluja, {Ashok K.}",
year = "2002",
month = "11",
language = "English (US)",
volume = "283",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "5 46-5",

}

TY - JOUR

T1 - Inhibition of cyclooxygenase-2 ameliorates the severity of pancreatitis and associated lung injury

AU - Song, Albert M.

AU - Bhagat, Lakshmi

AU - Singh, Vijay Prem

AU - Van Acker, Gijs G D

AU - Steer, Michael L.

AU - Saluja, Ashok K.

PY - 2002/11

Y1 - 2002/11

N2 - Cyclooxygenase-2 (COX-2), a widely distributed enzyme, plays an important role in inflammation. We have studied the role of COX-2 in acute pancreatitis and pancreatitis-associated lung injury using both the pharmacological inhibition of COX-2 and genetic deletion of COX-2. Pancreatitis was induced in mice by 12 hourly injections of cerulein. The severity of pancreatitis was assessed by measuring serum amylase, pancreatic trypsin activity, intrapancreatic sequestration of neutrophils, and acinar cell necrosis. The severity of lung injury was evaluated by measuring lactate dehydrogenase levels in the bronchoalveolar lavage fluid and by quantitating neutrophil sequestration in the lung. In both the pharmacologically inhibited and genetically altered mice, the severity of pancreatitis and pancreatitis-associated lung injury was reduced compared with the noninhibited strains of COX-2-sufficient mice. This reduction in injury indicates that COX-2 plays an important proinflammatory role in pancreatitis and its associated lung injury. Our findings support the concept that COX-2 inhibitors may play a beneficial role in the prevention of acute pancreatitis or in the reduction of its severity.

AB - Cyclooxygenase-2 (COX-2), a widely distributed enzyme, plays an important role in inflammation. We have studied the role of COX-2 in acute pancreatitis and pancreatitis-associated lung injury using both the pharmacological inhibition of COX-2 and genetic deletion of COX-2. Pancreatitis was induced in mice by 12 hourly injections of cerulein. The severity of pancreatitis was assessed by measuring serum amylase, pancreatic trypsin activity, intrapancreatic sequestration of neutrophils, and acinar cell necrosis. The severity of lung injury was evaluated by measuring lactate dehydrogenase levels in the bronchoalveolar lavage fluid and by quantitating neutrophil sequestration in the lung. In both the pharmacologically inhibited and genetically altered mice, the severity of pancreatitis and pancreatitis-associated lung injury was reduced compared with the noninhibited strains of COX-2-sufficient mice. This reduction in injury indicates that COX-2 plays an important proinflammatory role in pancreatitis and its associated lung injury. Our findings support the concept that COX-2 inhibitors may play a beneficial role in the prevention of acute pancreatitis or in the reduction of its severity.

KW - Cerulein

KW - Heat shock protein 70

KW - Inducible nitric oxide synthase

KW - Myeloperoxidase

KW - Neutrophil

KW - Proinflammatory

KW - Prostaglandin

KW - Trypsin

UR - http://www.scopus.com/inward/record.url?scp=0036839741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036839741&partnerID=8YFLogxK

M3 - Article

C2 - 12381531

AN - SCOPUS:0036839741

VL - 283

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 5 46-5

ER -