Inhibition controls for qualitative real-time PCR assays: Are they necessary for all specimen matrices?

S. P. Buckwalter, L. M. Sloan, S. A. Cunningham, M. J. Espy, J. R. Uhl, M. F. Jones, E. A. Vetter, Jayawant Mandrekar, F. R. Cockerill, B. S. Pritt, Robin Patel, N. L. Wengenack

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. Inhibition rates of ≤1% were found for all specimen matrix types except urine and formalin-fixed, paraffin-embedded tissue.

Original languageEnglish (US)
Pages (from-to)2139-2143
Number of pages5
JournalJournal of Clinical Microbiology
Volume52
Issue number6
DOIs
StatePublished - 2014

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Paraffin
Formaldehyde
Real-Time Polymerase Chain Reaction
Urine

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Buckwalter, S. P., Sloan, L. M., Cunningham, S. A., Espy, M. J., Uhl, J. R., Jones, M. F., ... Wengenack, N. L. (2014). Inhibition controls for qualitative real-time PCR assays: Are they necessary for all specimen matrices? Journal of Clinical Microbiology, 52(6), 2139-2143. https://doi.org/10.1128/JCM.03389-13

Inhibition controls for qualitative real-time PCR assays : Are they necessary for all specimen matrices? / Buckwalter, S. P.; Sloan, L. M.; Cunningham, S. A.; Espy, M. J.; Uhl, J. R.; Jones, M. F.; Vetter, E. A.; Mandrekar, Jayawant; Cockerill, F. R.; Pritt, B. S.; Patel, Robin; Wengenack, N. L.

In: Journal of Clinical Microbiology, Vol. 52, No. 6, 2014, p. 2139-2143.

Research output: Contribution to journalArticle

Buckwalter, SP, Sloan, LM, Cunningham, SA, Espy, MJ, Uhl, JR, Jones, MF, Vetter, EA, Mandrekar, J, Cockerill, FR, Pritt, BS, Patel, R & Wengenack, NL 2014, 'Inhibition controls for qualitative real-time PCR assays: Are they necessary for all specimen matrices?', Journal of Clinical Microbiology, vol. 52, no. 6, pp. 2139-2143. https://doi.org/10.1128/JCM.03389-13
Buckwalter, S. P. ; Sloan, L. M. ; Cunningham, S. A. ; Espy, M. J. ; Uhl, J. R. ; Jones, M. F. ; Vetter, E. A. ; Mandrekar, Jayawant ; Cockerill, F. R. ; Pritt, B. S. ; Patel, Robin ; Wengenack, N. L. / Inhibition controls for qualitative real-time PCR assays : Are they necessary for all specimen matrices?. In: Journal of Clinical Microbiology. 2014 ; Vol. 52, No. 6. pp. 2139-2143.
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