Abstract
A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. Inhibition rates of ≤1% were found for all specimen matrix types except urine and formalin-fixed, paraffin-embedded tissue.
Original language | English (US) |
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Pages (from-to) | 2139-2143 |
Number of pages | 5 |
Journal | Journal of clinical microbiology |
Volume | 52 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2014 |
ASJC Scopus subject areas
- Microbiology (medical)