Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1E-115)

F. Shintani, S. Kanba, T. Nakaki, R. Nakamura, K. Sato, G. Yagi, E. Richelson, R. Kato, M. Asai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, N1E-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [3H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 μM L-N(G)-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor-activated cyclic GMP formation in the cells. In addition, Li+ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalNeuropsychopharmacology
Volume11
Issue number2
StatePublished - 1994

Fingerprint

Cyclic GMP
Muscarinic Receptors
Neuroblastoma
Lithium
Guanylate Cyclase
Nitric Oxide
Nitrites
Nitrates
omega-N-Methylarginine
Nitric Oxide Donors
Nitroprusside
Nitric Oxide Synthase
Clone Cells
Ions

Keywords

  • Cyclic GMP
  • Lithium chloride
  • Muscarinic receptor
  • N1E-115 cells
  • Nitric oxide
  • Sodium nitroprusside

ASJC Scopus subject areas

  • Pharmacology

Cite this

Shintani, F., Kanba, S., Nakaki, T., Nakamura, R., Sato, K., Yagi, G., ... Asai, M. (1994). Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1E-115). Neuropsychopharmacology, 11(2), 119-124.

Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1E-115). / Shintani, F.; Kanba, S.; Nakaki, T.; Nakamura, R.; Sato, K.; Yagi, G.; Richelson, E.; Kato, R.; Asai, M.

In: Neuropsychopharmacology, Vol. 11, No. 2, 1994, p. 119-124.

Research output: Contribution to journalArticle

Shintani, F, Kanba, S, Nakaki, T, Nakamura, R, Sato, K, Yagi, G, Richelson, E, Kato, R & Asai, M 1994, 'Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1E-115)', Neuropsychopharmacology, vol. 11, no. 2, pp. 119-124.
Shintani, F. ; Kanba, S. ; Nakaki, T. ; Nakamura, R. ; Sato, K. ; Yagi, G. ; Richelson, E. ; Kato, R. ; Asai, M. / Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1E-115). In: Neuropsychopharmacology. 1994 ; Vol. 11, No. 2. pp. 119-124.
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