Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1e-115)

Futoshi Shintani; Shigenobu Kanba; Toshio Nakaki; Ryoko Nakamura; Koichi Sato; Gohei Yagi; Elliott Richelson; Ryuichi Kato; Masahiro Asai

doi:10.1038/npp.1994.41

Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1e-115)

Futoshi Shintani, Shigenobu Kanba, Toshio Nakaki, Ryoko Nakamura, Koichi Sato, Gohei Yagi, Elliott Richelson, Ryuichi Kato, Masahiro Asai

Neuroscience

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, NIE-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [³H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 11M L-NG-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor- activated cyclic GMP formation in the cells. In addition, Li⁺ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.

Original language	English (US)
Pages (from-to)	119-124
Number of pages	6
Journal	Neuropsychopharmacology
Volume	11
Issue number	2
DOIs	https://doi.org/10.1038/npp.1994.41
State	Published - Oct 1994

Keywords

Cyclic gmp
Lithium chloride
Muscarinic receptor
Nie-115 cells
Nitric oxide
Sodium nitroprusside

ASJC Scopus subject areas

Pharmacology
Psychiatry and Mental health

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10.1038/npp.1994.41

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title = "Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1e-115)",

abstract = "We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, NIE-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [3H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 11M L-NG-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor- activated cyclic GMP formation in the cells. In addition, Li+ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.",

keywords = "Cyclic gmp, Lithium chloride, Muscarinic receptor, Nie-115 cells, Nitric oxide, Sodium nitroprusside",

author = "Futoshi Shintani and Shigenobu Kanba and Toshio Nakaki and Ryoko Nakamura and Koichi Sato and Gohei Yagi and Elliott Richelson and Ryuichi Kato and Masahiro Asai",

note = "Funding Information: This work was supported in part by Keio Gijuku Academic Development Funds, a Research Grant for Nervous and Mental Disorders from the Ministry of Health and Welfare and a Research Grant from Ohme Keiyu Hospital (Director: Nobuo Otsuka, M.D.). Copyright: Copyright 2016 Elsevier B.V., All rights reserved.",

year = "1994",

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AU - Shintani, Futoshi

AU - Kanba, Shigenobu

AU - Nakaki, Toshio

AU - Nakamura, Ryoko

AU - Sato, Koichi

AU - Yagi, Gohei

AU - Richelson, Elliott

AU - Kato, Ryuichi

AU - Asai, Masahiro

N1 - Funding Information: This work was supported in part by Keio Gijuku Academic Development Funds, a Research Grant for Nervous and Mental Disorders from the Ministry of Health and Welfare and a Research Grant from Ohme Keiyu Hospital (Director: Nobuo Otsuka, M.D.). Copyright: Copyright 2016 Elsevier B.V., All rights reserved.

PY - 1994/10

Y1 - 1994/10

N2 - We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, NIE-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [3H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 11M L-NG-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor- activated cyclic GMP formation in the cells. In addition, Li+ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.

AB - We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, NIE-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [3H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 11M L-NG-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor- activated cyclic GMP formation in the cells. In addition, Li+ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.

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KW - Nie-115 cells

KW - Nitric oxide

KW - Sodium nitroprusside

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Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1e-115)

Abstract

Keywords

ASJC Scopus subject areas

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