Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1E-115)

F. Shintani, S. Kanba, T. Nakaki, R. Nakamura, K. Sato, G. Yagi, E. Richelson, R. Kato, M. Asai

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, N1E-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [3H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 μM L-N(G)-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor-activated cyclic GMP formation in the cells. In addition, Li+ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalNeuropsychopharmacology
Volume11
Issue number2
StatePublished - 1994

Keywords

  • Cyclic GMP
  • Lithium chloride
  • Muscarinic receptor
  • N1E-115 cells
  • Nitric oxide
  • Sodium nitroprusside

ASJC Scopus subject areas

  • Pharmacology

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    Shintani, F., Kanba, S., Nakaki, T., Nakamura, R., Sato, K., Yagi, G., Richelson, E., Kato, R., & Asai, M. (1994). Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1E-115). Neuropsychopharmacology, 11(2), 119-124.