Abstract
Whole-cell recordings were performed on rat trigeminal ganglion (TG) neurons as a modeling experiment to investigate the effect of bis (7)-tacrine, a potential anti-Alzheimer's disease (AD) drug, on 5-HT-induced current (I 5-HT). Extracellular 5-HT activated a concentration-dependent inward current that was blocked by ICS 205930. Co-application of bis(7)-tacrine inhibited I5-HT markedly with IC50 at 2 × 10 -6 M. Bis(7)-tacrine shifted the concentration-response curve for I5-HT rightwards with its maximum response unchanged and EC 50 increased, suggesting that this inhibition was competitive in nature. Intracellular dialysis of GDP-β-S did not block bis(7)-tacrine inhibition of I5-HT, which excluded the involvement of G-protein mediation. These results may offer possible modality to understanding the anti-AD mechanism of bis(7)-tacrine.
Original language | English (US) |
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Pages (from-to) | 1335-1338 |
Number of pages | 4 |
Journal | NeuroReport |
Volume | 15 |
Issue number | 8 |
DOIs | |
State | Published - Jun 7 2004 |
Keywords
- 5-HT receptor
- Bis(7)-tacrine
- Current
- Inhibition
- Rat
- Trigeminal ganglion
ASJC Scopus subject areas
- General Neuroscience