Inhalation of carbon monoxide reduces skeletal muscle injury after hind limb ischemia-reperfusion injury in mice

Rajendra Patel, Hassan Albadawi, Wolfgang Steudel, Faraz F. Hashmi, Jeanwan Kang, Hyung Jin Yoo, Michael T. Watkins

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: The purpose of this study was to determine if inhaled carbon monoxide (CO) can ameliorate skeletal muscle injury, modulate endogenous heme oxygenase-1 expression, and improve indexes of tissue integrity and inflammation after hind limb ischemia reperfusion. Methods: C57BL6 mice inhaling CO (250 ppm) or room air were subjected to 1.5 hours of ischemia followed by limb reperfusion for either 3 or 6 hours (total treatment time, 4.5 or 7.5 h). After the initial period of reperfusion, all mice breathed only room air until 24 hours after the onset of ischemia. Mice were killed at either the end of CO treatment or at 24 hours' reperfusion. Skeletal muscle was subjected to histologic and biochemical analysis. Results: CO treatment for 7.5 hours protected skeletal muscle from histologic and structural evidence of skeletal muscle injury. Serum and tissue cytokines were reduced significantly (P <.05) in mice treated with CO for 7.5 hours. Tubulin, heme oxygenase, and adenosine triphosphate levels were higher in CO-treated mice. Conclusions: Inhaled CO protected muscle from structural injury and energy depletion after ischemia reperfusion.

Original languageEnglish (US)
Pages (from-to)488-495
Number of pages8
JournalAmerican Journal of Surgery
Volume203
Issue number4
DOIs
StatePublished - Apr 1 2012
Externally publishedYes

Fingerprint

Carbon Monoxide
Reperfusion Injury
Inhalation
Skeletal Muscle
Extremities
Reperfusion
Wounds and Injuries
Ischemia
Air
Heme Oxygenase (Decyclizing)
Heme Oxygenase-1
Tubulin
Adenosine Triphosphate
Cytokines
Inflammation
Muscles
Serum

Keywords

  • Carbon monoxide
  • Heme oxygenase
  • Reperfusion injury
  • Skeletal muscle

ASJC Scopus subject areas

  • Surgery

Cite this

Inhalation of carbon monoxide reduces skeletal muscle injury after hind limb ischemia-reperfusion injury in mice. / Patel, Rajendra; Albadawi, Hassan; Steudel, Wolfgang; Hashmi, Faraz F.; Kang, Jeanwan; Yoo, Hyung Jin; Watkins, Michael T.

In: American Journal of Surgery, Vol. 203, No. 4, 01.04.2012, p. 488-495.

Research output: Contribution to journalArticle

Patel, Rajendra ; Albadawi, Hassan ; Steudel, Wolfgang ; Hashmi, Faraz F. ; Kang, Jeanwan ; Yoo, Hyung Jin ; Watkins, Michael T. / Inhalation of carbon monoxide reduces skeletal muscle injury after hind limb ischemia-reperfusion injury in mice. In: American Journal of Surgery. 2012 ; Vol. 203, No. 4. pp. 488-495.
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