Influenza virus neuraminidase alters aiiogeneic T cell proliferation

SangKon Oh, Maryna C. Eichelberger

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Influenza virus elicits good cellular and humoral immune responses. Unlike the cytotoxic T lymphocyte response to many other antigens, the cytotoxic T lymphocyte response to influenza virus is CD4+ T cell independent, suggesting that viral infection of antigen-presenting cells may alter their capacity to stimulate T cell responses. To clarify the role of influenza virus in these functional alterations, we compared T cell responses to uninfected and A/PR/8/34-infected dendritic cells (DC). DC were prepared from the bone marrow of C57BL/6 (H2b) mice and used to stimulate in vivo and in vitro alloreactive T cell responses. In both cases, influenza virus infection increased the capacity of DC to stimulate T cell proliferation. This enhancement was blocked by antibodies specific for neuraminidase (NA), but not hemagglutinin. Infection was not required to observe enhanced T cell proliferation, showing that NA from exogeneous virus particles can facilitate this effect. These results are the first to show that influenza virus alters the capacity of DC to stimulate T cell proliferation through mechanisms mediated by viral NA.

Original languageEnglish (US)
Pages (from-to)427-435
Number of pages9
JournalVirology
Volume264
Issue number2
DOIs
StatePublished - Nov 25 1999
Externally publishedYes

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ASJC Scopus subject areas

  • Virology

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