Influencing factors on the NMP-22 urine assay: An experimental model

Makito Miyake, Steve Goodison, Evan Gomes Giacoia, Wasia Rizwani, Shanti Ross, Charles J. Rosser

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Background: The commercial NMP-22 urine assays for bladder cancer (BCa) detect nuclear mitotic apparatus protein 1 (NUMA1) using monoclonal antibodies. It remains unclear whether these assays are monitoring a tumor antigen or some other phenomenon associated with the disease state. In this study, we investigated the influence of urinary cellular and protein concentration, and hematuria on the performance of the NMP-22 tests in an experimental model. Methods. Pooled urine from healthy subjects were spiked with varying concentrations of benign (UROtsa) cells, cancer cells (RT4, T24, KU-7 and UM-UC-14), whole blood or serum, prior to analysis with both NMP22 Bladder Cancer ELISA test and the NMP22 BladderChek point-of-care test. Results: Urines from control subjects were negative for NMP-22. The addition of whole blood at 50ul/10ml, but not serum, resulted in a false-positive result. Furthermore, the addition of a high concentration of benign urothelial cells (106) or the cell lysate from these cells (306g protein) resulted in a false-positive result. High concentrations of pooled-cancer cells (106) or cell lysate (30.6g and above) resulted in a positive NMP-22 assay. Concordance between the NMP-22 ELISA assay and the NMP-22 point of care assay was >90%. Conclusions: Rather than detecting a specific tumor antigen, urinary NMP-22 assays may be measuring the cellularity or amount of cell turnover that may be introduced into the urine by a variety of conditions, including surface shedding from bladder tumors. The absence of significant urinary cellularity in some cases due to lesion characteristics or the timing of sampling may result in false-negative NMP-2 assays.

Original languageEnglish (US)
Article number23
JournalBMC Urology
Volume12
DOIs
StatePublished - 2012

Keywords

  • Bladder cancer
  • NMP-22
  • Urine

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

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    Miyake, M., Goodison, S., Giacoia, E. G., Rizwani, W., Ross, S., & Rosser, C. J. (2012). Influencing factors on the NMP-22 urine assay: An experimental model. BMC Urology, 12, [23]. https://doi.org/10.1186/1471-2490-12-23