Influence of scatter factor/hepatocyte growth factor on the growth and transmission of hepatocarcinoma SMMC 7721 cells

Q. Xie, K. Liu, M. Hu, X. Wu, K. Zhou

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

OBJECTIVE: To explore the influence of scatter factor/hepatocyte growth factor (SF/HGF) on the growth and transmission of hepatocarcinoma SMMC 7721 cells by SF/HGF cDNA transfection. METHODS: Gene transfection was operated by lipofectin method. In vitro, SF/HGF and c-met expression was tested by ELISA and Western blot. Cell proliferation and motility were compared by growth curves and wound healing assays. In vivo, cells before and after transfection were implanted subcutaneously into nude mice respectively to observe tumor growth and metastasis. RESULTS: After transfection, the expression of SF/HGF reached 694 pg/ml, compared with 0.26 pg/ml before transfection; however the expression of c-met protein did not change obviously. Growth curves showed that cells after transfection proliferated more rapidly than those before transfection and had an increased capability of motility together with enlarged morphological changes. In vivo experiment, tumors originated from SF/HGF(+) cells grew faster than those from SF/HGF(-) cells and had embolism formed inside and metastasis in the corresponding lung tissues, whereas no such findings in SF/HGF(-) cell originated tumors. CONCLUSIONS: High expression of SF/HGF can stimulate tumor cell proliferation and metastasis in hepatocellular carcinoma

Original languageEnglish (US)
Pages (from-to)95-97
Number of pages3
JournalZhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Volume9
Issue number2
StatePublished - Apr 2001

ASJC Scopus subject areas

  • Medicine(all)

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