Influence of hyaluronic acid transitions in tumor microenvironment on glioblastoma malignancy and invasive behavior

Jee Wei E. Chen, Sara Pedron, Peter Shyu, Yuhang Hu, Jann N Sarkaria, Brendan A.C. Harley

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The extracellular matrix (ECM) is critical in tumor growth and invasive potential of cancer cells. In glioblastoma tumors, some components of the native brain ECM such as hyaluronic acid (HA) have been suggested as key regulators of processes associated with poor patient outlook such as invasion and therapeutic resistance. Given the importance of cell-mediated remodeling during invasion, it is likely that the molecular weight of available HA polymer may strongly influence GBM progression. Biomaterial platforms therefore provide a unique opportunity to systematically examine the influence of the molecular weight distribution of HA on GBM cell activity. Here we report the relationship between the molecular weight of matrix-bound HA within a methacrylamide-functionalized gelatin (GelMA) hydrogel, the invasive phenotype of a patient-derived xenograft GBM population that exhibits significant in vivo invasivity, and the local production of soluble HA during GBM cell invasion. Hyaluronic acid of different molecular weights spanning a range associated with cell-mediated remodeling (10, 60, and 500 kDa) was photopolymerized into GelMA hydrogels, with cell activity compared to GelMA only conditions (-HA). Polymerization conditions were tuned to create a homologous series of GelMA hydrogels with conserved poroelastic properties (i.e., shear modulus, Poisson's ratio, and diffusivity). GBM migration was strongly influenced by HA molecular weight; while markers associated with active remodeling of HA (hyaluronan synthase and hyaluronidase) were found to be uninfluenced. These results provide new information regarding the importance of local hyaluronic acid content on the invasive phenotype of GBM.

Original languageEnglish (US)
Article number39
JournalFrontiers in Materials
Volume5
DOIs
StatePublished - Jun 26 2018

Fingerprint

Hyaluronic acid
Hyaluronic Acid
Tumors
Molecular weight
Hydrogels
Hyaluronoglucosaminidase
Hydrogel
Biocompatible Materials
Poisson ratio
Gelatin
Molecular weight distribution
Heterografts
Biomaterials
Brain
Polymers
Elastic moduli
Polymerization
Cells

Keywords

  • Brain tumor
  • Cell invasion
  • Glioblastoma
  • Hyaluronic acid
  • Hydrogels
  • Molecular weight
  • Tumor margins
  • Tumor microenvironment

ASJC Scopus subject areas

  • Materials Science (miscellaneous)

Cite this

Influence of hyaluronic acid transitions in tumor microenvironment on glioblastoma malignancy and invasive behavior. / Chen, Jee Wei E.; Pedron, Sara; Shyu, Peter; Hu, Yuhang; Sarkaria, Jann N; Harley, Brendan A.C.

In: Frontiers in Materials, Vol. 5, 39, 26.06.2018.

Research output: Contribution to journalArticle

Chen, Jee Wei E. ; Pedron, Sara ; Shyu, Peter ; Hu, Yuhang ; Sarkaria, Jann N ; Harley, Brendan A.C. / Influence of hyaluronic acid transitions in tumor microenvironment on glioblastoma malignancy and invasive behavior. In: Frontiers in Materials. 2018 ; Vol. 5.
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