Influence of host genetic variation on rubella-specific T cell cytokine responses following rubella vaccination

Inna G. Ovsyannikova; Jenna E. Ryan; Robert A. Vierkant; Megan M. O'Byrne; V. Shane Pankratz; Robert M. Jacobson; Gregory A. Poland

doi:10.1016/j.vaccine.2009.01.079

Influence of host genetic variation on rubella-specific T cell cytokine responses following rubella vaccination

Inna G. Ovsyannikova, Jenna E. Ryan, Robert A. Vierkant, Megan M. O'Byrne, V. Shane Pankratz, Robert M. Jacobson, Gregory A. Poland

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The variability of immune response modulated by immune response gene polymorphisms is a significant factor in the protective effect of vaccines. We studied the association between cellular (cytokine) immunity and HLA genes among 738 schoolchildren (396 males and 342 females) between the ages of 11 and 19 years, who received two doses of rubella vaccine (Merck). Cytokine secretion levels in response to rubella virus stimulation were determined in PBMC cultures by ELISA. Cell supernatants were assayed for Th1 (IFN-γ, IL-2, and IL-12p40), Th2 (IL-4, IL-5, and IL-10), and innate/proinflammatory (TNF-α, GM-CSF, and IL-6) cytokines. We found a strong association between multiple alleles of the HLA-DQA1 (global p-value 0.022) and HLA-DQB1 (global p-value 0.007) loci and variations in rubella-specific IL-2 cytokine secretion. Additionally, the relationships between alleles of the HLA-A (global p-value 0.058), HLA-B (global p-value 0.035), and HLA-C (global p-value 0.023) loci and TNF-α secretion suggest the importance of HLA class I molecules in innate/inflammatory immune response. Better characterization of these genetic profiles could help to predict immune responses at the individual and population level, provide data on mechanisms of immune response development, and further inform vaccine development and vaccination policies.

Original language	English (US)
Pages (from-to)	3359-3366
Number of pages	8
Journal	Vaccine
Volume	27
Issue number	25-26
DOIs	https://doi.org/10.1016/j.vaccine.2009.01.079
State	Published - May 26 2009

Keywords

Cellular responses
Cytokines
ELISA
ELISPOT
HLA alleles
Rubella vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2009.01.079

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@article{b9b641901e1a43449bb4c2c225888cb5,

title = "Influence of host genetic variation on rubella-specific T cell cytokine responses following rubella vaccination",

abstract = "The variability of immune response modulated by immune response gene polymorphisms is a significant factor in the protective effect of vaccines. We studied the association between cellular (cytokine) immunity and HLA genes among 738 schoolchildren (396 males and 342 females) between the ages of 11 and 19 years, who received two doses of rubella vaccine (Merck). Cytokine secretion levels in response to rubella virus stimulation were determined in PBMC cultures by ELISA. Cell supernatants were assayed for Th1 (IFN-γ, IL-2, and IL-12p40), Th2 (IL-4, IL-5, and IL-10), and innate/proinflammatory (TNF-α, GM-CSF, and IL-6) cytokines. We found a strong association between multiple alleles of the HLA-DQA1 (global p-value 0.022) and HLA-DQB1 (global p-value 0.007) loci and variations in rubella-specific IL-2 cytokine secretion. Additionally, the relationships between alleles of the HLA-A (global p-value 0.058), HLA-B (global p-value 0.035), and HLA-C (global p-value 0.023) loci and TNF-α secretion suggest the importance of HLA class I molecules in innate/inflammatory immune response. Better characterization of these genetic profiles could help to predict immune responses at the individual and population level, provide data on mechanisms of immune response development, and further inform vaccine development and vaccination policies.",

keywords = "Cellular responses, Cytokines, ELISA, ELISPOT, HLA alleles, Rubella vaccine",

author = "Ovsyannikova, {Inna G.} and Ryan, {Jenna E.} and Vierkant, {Robert A.} and O'Byrne, {Megan M.} and Pankratz, {V. Shane} and Jacobson, {Robert M.} and Poland, {Gregory A.}",

note = "Funding Information: We thank the Mayo Vaccine Research Group and subjects who participated in our studies. We thank Cheri A. Hart for her editorial assistance. This work was supported by NIH grants AI 48793, AI 33144 and 1 UL1 RR024150-01 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health, and the NIH Roadmap for Medical Research. Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH. ",

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doi = "10.1016/j.vaccine.2009.01.079",

language = "English (US)",

volume = "27",

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AU - Ovsyannikova, Inna G.

AU - Ryan, Jenna E.

AU - Vierkant, Robert A.

AU - O'Byrne, Megan M.

AU - Pankratz, V. Shane

AU - Jacobson, Robert M.

AU - Poland, Gregory A.

N1 - Funding Information: We thank the Mayo Vaccine Research Group and subjects who participated in our studies. We thank Cheri A. Hart for her editorial assistance. This work was supported by NIH grants AI 48793, AI 33144 and 1 UL1 RR024150-01 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health, and the NIH Roadmap for Medical Research. Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH.

PY - 2009/5/26

Y1 - 2009/5/26

N2 - The variability of immune response modulated by immune response gene polymorphisms is a significant factor in the protective effect of vaccines. We studied the association between cellular (cytokine) immunity and HLA genes among 738 schoolchildren (396 males and 342 females) between the ages of 11 and 19 years, who received two doses of rubella vaccine (Merck). Cytokine secretion levels in response to rubella virus stimulation were determined in PBMC cultures by ELISA. Cell supernatants were assayed for Th1 (IFN-γ, IL-2, and IL-12p40), Th2 (IL-4, IL-5, and IL-10), and innate/proinflammatory (TNF-α, GM-CSF, and IL-6) cytokines. We found a strong association between multiple alleles of the HLA-DQA1 (global p-value 0.022) and HLA-DQB1 (global p-value 0.007) loci and variations in rubella-specific IL-2 cytokine secretion. Additionally, the relationships between alleles of the HLA-A (global p-value 0.058), HLA-B (global p-value 0.035), and HLA-C (global p-value 0.023) loci and TNF-α secretion suggest the importance of HLA class I molecules in innate/inflammatory immune response. Better characterization of these genetic profiles could help to predict immune responses at the individual and population level, provide data on mechanisms of immune response development, and further inform vaccine development and vaccination policies.

AB - The variability of immune response modulated by immune response gene polymorphisms is a significant factor in the protective effect of vaccines. We studied the association between cellular (cytokine) immunity and HLA genes among 738 schoolchildren (396 males and 342 females) between the ages of 11 and 19 years, who received two doses of rubella vaccine (Merck). Cytokine secretion levels in response to rubella virus stimulation were determined in PBMC cultures by ELISA. Cell supernatants were assayed for Th1 (IFN-γ, IL-2, and IL-12p40), Th2 (IL-4, IL-5, and IL-10), and innate/proinflammatory (TNF-α, GM-CSF, and IL-6) cytokines. We found a strong association between multiple alleles of the HLA-DQA1 (global p-value 0.022) and HLA-DQB1 (global p-value 0.007) loci and variations in rubella-specific IL-2 cytokine secretion. Additionally, the relationships between alleles of the HLA-A (global p-value 0.058), HLA-B (global p-value 0.035), and HLA-C (global p-value 0.023) loci and TNF-α secretion suggest the importance of HLA class I molecules in innate/inflammatory immune response. Better characterization of these genetic profiles could help to predict immune responses at the individual and population level, provide data on mechanisms of immune response development, and further inform vaccine development and vaccination policies.

KW - Cellular responses

KW - Cytokines

KW - ELISA

KW - ELISPOT

KW - HLA alleles

KW - Rubella vaccine

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DO - 10.1016/j.vaccine.2009.01.079

M3 - Article

C2 - 19200845

AN - SCOPUS:67349268939

SN - 0264-410X

VL - 27

SP - 3359

EP - 3366

JO - Vaccine

JF - Vaccine

IS - 25-26

ER -

Influence of host genetic variation on rubella-specific T cell cytokine responses following rubella vaccination

Abstract

Keywords

ASJC Scopus subject areas

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