Influence of HLA-DRB1 alleles on lymphoproliferative responses to a naturally processed and presented measles virus phosphoprotein in measles immunized individuals

Inna G. Ovsyannikova, Gregory A. Poland, Nathan J. Easler, Robert A. Vierkant

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Identification of stimulatory T-cell epitopes recognized by CD4 + T lymphocytes is important for vaccine development. Our previous studies using mass spectrometry identified a naturally processed HLA class II restricted DRB1*0301 T cell epitope in the measles virus phosphoprotein, MV-P1 (residues 179-197). Here we provide lymphocyte proliferation data from peripheral blood mononuclear cells (PBMC) obtained from 131 HLA-DRB1*0301- positive and HLA-DRB1*0301-negative (HLA discordant) individuals previously immunized against measles and report that a single amino acid substitution in the MV-P1 T cell epitope can reduce T cell proliferation and CD4+ T-cell recognition. Measles virus and measles peptide-specific lymphoproliferative responses and HLA-DRB1 allele associations reveal that the DRB1*0701 allele provided suggestive evidence of association with both measles virus (p = 0.03) and MV-P1 peptide (p = 0.06) lymphoproliferation. A marginally significant increase in the frequency of the*0301 allele (p = 0.10) was found among subjects who demonstrated low cellular responses to the measles virus. We found no associations between proliferation levels to the MV-P1 and MV-P2 peptides with*0301 alleles. We speculate that the glutamic acid at position 192 of the measles phosphoprotein is a critical immunogenicity factor and may influence the antigenicity of the naturally processed HLA class II MV-P1 epitope.

Original languageEnglish (US)
Pages (from-to)209-217
Number of pages9
JournalHuman Immunology
Volume65
Issue number3
DOIs
StatePublished - Mar 2004

Keywords

  • Cellular immunity
  • HLA class II epitopes
  • Measles
  • Measles peptides
  • T-cell recognition

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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