Influence of basement membrane proteins and endothelial cell-derived factors on the morphology of human fetal-derived astrocytes in 2D

Amanda F. Levy, Maya Zayats, Hugo Guerrero Cazares, Alfredo Quinones-Hinojosa, Peter C. Searson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Astrocytes are the most prevalent type of glial cell in the brain, participating in a variety of diverse functions from regulating cerebral blood flow to controlling synapse formation. Astrocytes and astrocyte-conditioned media are widely used in models of the blood-brain barrier (BBB), however, very little is known about astrocyte culture in 2D. To test the hypothesis that surface coating and soluble factors influence astrocyte morphology in 2D, we quantitatively analyzed the morphology of human fetal derived astrocytes on glass, matrigel, fibronectin, collagen IV, and collagen I, and after the addition soluble factors including platelet-derived growth factor (PDGF), laminin, basic fibroblast growth factor (bFGF), and leukemia inhibitory factor (LIF). Matrigel surface coatings, as well as addition of leukemia inhibitory factor (LIF) to the media, were found to have the strongest effects on 2D astrocyte morphology, and may be important in improving existing BBB models. In addition, the novel set of quantitative parameters proposed in this paper provide a test for determining the influence of compounds on astrocyte morphology, both to screen for new endothelial cell-secreted factors that influence astrocytes, and to determine in a high-throughput way which factors are important for translation to more complex, 3D BBB models.

Original languageEnglish (US)
Article numbere92165
JournalPLoS One
Volume9
Issue number3
DOIs
StatePublished - Mar 19 2014
Externally publishedYes

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astrocytes
Endothelial cells
basement membrane
Basement Membrane
membrane proteins
Astrocytes
endothelial cells
Membrane Proteins
Endothelial Cells
blood-brain barrier
Blood-Brain Barrier
Leukemia Inhibitory Factor
coatings
collagen
Cerebrovascular Circulation
Collagen
platelet-derived growth factor
fibroblast growth factor 2
Coatings
laminin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Influence of basement membrane proteins and endothelial cell-derived factors on the morphology of human fetal-derived astrocytes in 2D. / Levy, Amanda F.; Zayats, Maya; Guerrero Cazares, Hugo; Quinones-Hinojosa, Alfredo; Searson, Peter C.

In: PLoS One, Vol. 9, No. 3, e92165, 19.03.2014.

Research output: Contribution to journalArticle

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