TY - JOUR
T1 - Influence of asthma status on serotype-specific pneumococcal antibody levels
AU - Jung, Ji A.
AU - Kita, Hirohito
AU - Dhillon, Ravneet
AU - Jacobson, Robert M.
AU - Nahm, Moon H.
AU - Park, Miguel
AU - Tsigrelis, Constantine
AU - Juhn, Young J.
N1 - Funding Information:
We wish to thank Diane Squillace and Gail Kephardt at the Mayo Clinic and Jigui Yu at the University of Alabama, Birmingham, for their technical assistance on this study. We are indebted to Dr Roshini Abraham, Dr David Briles, and Dr Susan Hollingshead at the University of Alabama, Birmingham for their advice and comments on this study. We are also grateful for support from the staff of the Pediatric Asthma Epidemiology Research Unit and Clinical Research Unit at the Mayo Clinic. The work was supported by the Bridge Award from the Mayo Clinic.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010/9
Y1 - 2010/9
N2 - Background: Asthma has been reported to be associated with an increased risk of invasive pneumococcal disease (IPD). Objective: We compared serotype-specific antibody responses with pneumococcal polysaccharide antigens of individuals with and without asthma. Methods: A cross-sectional study was conducted for 16 subjects with asthma and 14 subjects without asthma from the community of Rochester, MN. Asthma was determined by predetermined criteria based on comprehensive medical record reviews. Serotype-specific antibody to 23 pneumococcal polysaccharide antigens was measured by enzyme-linked immunosorbent assay, and seropositivity was considered ≥ 1.3 μg/mL. Interferon-γ (IFN-γ) and interleukin-5 (IL-5) were measured from peripheral blood mononuclear cells cultured with house dust mites and staphylococcal enterotoxin B. Results: Of the 30 subjects, 16 (53%) were male, 21 (70%) were white, and the median age was 26 years. The median numbers of positive serotype-specific antibodies for asthmatics and nonasthmatics were 8.5 and 15.5, respectively (P = 0.034). There was an inverse relationship between the ratio of log-transformed IL-5/IFN-γ and the number of positive serotype-specific antibodies (r = -0.36; P = 0.052). As potential covariates and confounders, a history of pneumococcal vaccination (P = 0.84), having a high-risk condition for IPD (P = 0.68), and taking asthma medications, including inhaled/systemic corticosteroids (P = 0.79), were not associated with the number of positive serotype-specific antibodies. Conclusion: Asthmatics had significantly lower serotype-specific pneumococcal antibody levels than nonasthmatics. House dust mite-induced T-helper 2 (Th2) cytokine immune profile may be related to the association. This may account for an increased risk of IPD in asthmatics and deserves further investigation.
AB - Background: Asthma has been reported to be associated with an increased risk of invasive pneumococcal disease (IPD). Objective: We compared serotype-specific antibody responses with pneumococcal polysaccharide antigens of individuals with and without asthma. Methods: A cross-sectional study was conducted for 16 subjects with asthma and 14 subjects without asthma from the community of Rochester, MN. Asthma was determined by predetermined criteria based on comprehensive medical record reviews. Serotype-specific antibody to 23 pneumococcal polysaccharide antigens was measured by enzyme-linked immunosorbent assay, and seropositivity was considered ≥ 1.3 μg/mL. Interferon-γ (IFN-γ) and interleukin-5 (IL-5) were measured from peripheral blood mononuclear cells cultured with house dust mites and staphylococcal enterotoxin B. Results: Of the 30 subjects, 16 (53%) were male, 21 (70%) were white, and the median age was 26 years. The median numbers of positive serotype-specific antibodies for asthmatics and nonasthmatics were 8.5 and 15.5, respectively (P = 0.034). There was an inverse relationship between the ratio of log-transformed IL-5/IFN-γ and the number of positive serotype-specific antibodies (r = -0.36; P = 0.052). As potential covariates and confounders, a history of pneumococcal vaccination (P = 0.84), having a high-risk condition for IPD (P = 0.68), and taking asthma medications, including inhaled/systemic corticosteroids (P = 0.79), were not associated with the number of positive serotype-specific antibodies. Conclusion: Asthmatics had significantly lower serotype-specific pneumococcal antibody levels than nonasthmatics. House dust mite-induced T-helper 2 (Th2) cytokine immune profile may be related to the association. This may account for an increased risk of IPD in asthmatics and deserves further investigation.
KW - Asthma
KW - Epidemiology
KW - Humoral immunity
KW - PPV23
KW - Pneumococcal pneumonia
KW - Pneumococcal polysaccharide antigens
KW - Risk
KW - Rochester Epidemiology Project
KW - Serotype-specific pneumococcal antibody
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U2 - 10.3810/pgm.2010.09.2208
DO - 10.3810/pgm.2010.09.2208
M3 - Review article
C2 - 20861595
AN - SCOPUS:78049499520
SN - 0032-5481
VL - 122
SP - 116
EP - 124
JO - Postgraduate medicine
JF - Postgraduate medicine
IS - 5
ER -