Influence of δ-opioid receptors on production of labeled methionine5-enkephalin in murine neuroblastoma cells

J. A. Gilbert, E. Richelson

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Synthesis of methionine5-enkephalin by intact cells of murine neuroblastoma clone N1E-115 has been demonstrated both immunocytochemically and biochemically. In addition, N1E-115 cells possess homogeneous enkephalin (δ) receptors which inhibit prostaglandin E1-induced intracellular cyclic AMP formation. An assay was developed for measuring de novo synthesis of methionine5-enkephalin by pulsing cells in culture with radioactive methionine and isolating this pentapeptide to radiochemical purity by a procedure that included immunoaffinity chromatography specific for oxidized methionine5-enkephalin. This assay indicated that production of radiolabeled-methionine5-enkephalin was increased upon lengthy exposure of intact N1E-115 cells in the late logarithmic phase of growth to a nonproteolyzable analog of methionine5-enkephalin. This increase in synthesis of intracellular methionine5-enkephalin relative to control cells was prevented by prior incubation of the clone with naloxone, indicating that the response was mediated by the δ receptor.

Original languageEnglish (US)
Pages (from-to)922-928
Number of pages7
JournalJournal of Neurochemistry
Volume44
Issue number3
StatePublished - 1985

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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