Infection with a babesia-like organism (WA1) in selected strains of laboratory mice

Manuel Moro, Chella David, David Persing

Research output: Contribution to journalArticle

Abstract

A newly identified intraerythrocytic babesia-liko organism, causes infection in humans in the Western U.S. C57BL/10), BALB/c and C3H.RKK mice were inoculated intra- peritoneally with WA1 parasitized rod blood cells (PRBC). Parasitemia was estimated by couating PRBC on Giemsa-stained mouse blood smears collected at 3 day intervals after initial inoculation. C57BL/10 mice exhibited a 20% peak para parasitemia 14 days after inoculation and subsequen- tly recovered naturally from their primary infection. BALB/c mice exhibited a 30% peak parasitemia and 40% of the animals die 14-16 days after inoculation. C3H.RKK exhibited a 40% peak parasitemia. Over 95% of these animals die 14-16 days after inoculation. Red blood cell counts and hematocrit values indicated a severe anemia Also to determine whether major histocompatibility complex (MHC) genes affected the susceptibility to this parasite, we compared congenic mice differing only at the MHC. C57BL/10(H-2b, B10.BR(H-2k.C3H.RKK(H-2k and C3H.SW(H-2b were used in this study. B10.BR(H-2k mice developed a similar peak parasitemia and subsequent outcome as C57BL/10(H-2b. However C3H.SW(H-2b developed a 20% peak parasitemia and all animals recovered naturally in contrast to C3H.RKK(H-2k. These initial results indicate that susceptibility to WA1 is not only strain specific but also dependent of MHC haplotypes.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

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Babesia
Parasitemia
parasitemia
Blood
Animals
organisms
mice
major histocompatibility complex
Infection
Major Histocompatibility Complex
infection
Cells
blood cells
Blood Cells
Congenic Mice
animals
Erythrocyte Count
Genes
erythrocyte count
Inbred C57BL Mouse

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Infection with a babesia-like organism (WA1) in selected strains of laboratory mice. / Moro, Manuel; David, Chella; Persing, David.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Moro, Manuel ; David, Chella ; Persing, David. / Infection with a babesia-like organism (WA1) in selected strains of laboratory mice. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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