Infection of primary human macrophages with hepatitis C virus in vitro: Induction of tumour necrosis factor-α and interleukin 8

Marek Radkowski, Agnieszka Bednarska, Andrzej Horban, Janusz Stanczak, Jeffrey Wilkinson, Debra M. Adair, Marek Nowicki, Jorge Rakela, Tomasz Laskus

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Hepatitis C virus (HCV) has been reported to replicate in monocytes/macrophages in infected patients. However, it is unclear whether macrophages are susceptible to infection in vitro and whether such an infection is consequential. Sera from 26 HCV-infected patients were incubated with primary human macrophages collected from healthy donors. Virus negative strand was detected by a Tth enzyme-based strand-specific assay and virus sequences were analysed by single strand conformation polymorphism (SSCP) and sequencing. Concentrations of the cytokines tumour necrosis factor-α (TNF-α) and interleukin (IL)-1β, IL-6, IL-8, IL-10 and IL-12p70 were measured in culture supernatants and respective mRNAs were analysed in cell extracts by quantitative RT-PCR. For 15 sera, HCV RNA was detectable in 2- and 3-week cultures from at least one donor. Virus negative strand was detected in 29% of macrophage samples in this group. In four cases, HCV RNA sequences amplified from macrophages differed from those amplified from sera suggesting evolution during infection. Concentrations of TNF-α and IL-8 were found to be significantly higher in supernatants from HCV-infected cultures. In conclusion, these preliminary data suggest that primary human macrophages are susceptible to HCV infection in vitro and this infection is associated with the induction of cytokines TNF-α and IL-8.

Original languageEnglish (US)
Pages (from-to)47-59
Number of pages13
JournalJournal of General Virology
Volume85
Issue number1
DOIs
StatePublished - Jan 2004

ASJC Scopus subject areas

  • Virology

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