TY - JOUR
T1 - Infection of naïve target cells with virus-like particles
T2 - Implications for the function of ebola virus VP24
AU - Hoenen, Thomas
AU - Groseth, Allison
AU - Kolesnikova, Larissa
AU - Theriault, Steven
AU - Ebihara, Hideki
AU - Hartlieb, Bettina
AU - Bamberg, Sandra
AU - Feldmann, Heinz
AU - Ströher, Ute
AU - Becker, Stephan
PY - 2006/7
Y1 - 2006/7
N2 - Infectious virus-like particle (iVLP) systems have recently been established for several negative-strand RNA viruses, including the highly pathogenic Zaire ebolavirus (ZEBOV), and allow study of the viral life cycle under biosafety level 2 conditions. However, current systems depend on the expression of viral helper nucleocapsid proteins in target cells, thus making it impossible to determine whether ribonucleoprotein complexes transferred by iVLPs are able to facilitate initial transcription, an indispensable step in natural infection. Here we describe a ZEBOV iVLP system which overcomes this limitation and show that VP24 is essential for the formation of a functional ribonucleoprotein complex.
AB - Infectious virus-like particle (iVLP) systems have recently been established for several negative-strand RNA viruses, including the highly pathogenic Zaire ebolavirus (ZEBOV), and allow study of the viral life cycle under biosafety level 2 conditions. However, current systems depend on the expression of viral helper nucleocapsid proteins in target cells, thus making it impossible to determine whether ribonucleoprotein complexes transferred by iVLPs are able to facilitate initial transcription, an indispensable step in natural infection. Here we describe a ZEBOV iVLP system which overcomes this limitation and show that VP24 is essential for the formation of a functional ribonucleoprotein complex.
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U2 - 10.1128/JVI.00051-06
DO - 10.1128/JVI.00051-06
M3 - Article
C2 - 16809331
AN - SCOPUS:33745764575
SN - 0022-538X
VL - 80
SP - 7260
EP - 7264
JO - Journal of virology
JF - Journal of virology
IS - 14
ER -