Infection of naïve target cells with virus-like particles: Implications for the function of ebola virus VP24

Thomas Hoenen, Allison Groseth, Larissa Kolesnikova, Steven Theriault, Hideki Ebihara, Bettina Hartlieb, Sandra Bamberg, Heinz Feldmann, Ute Ströher, Stephan Becker

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

Infectious virus-like particle (iVLP) systems have recently been established for several negative-strand RNA viruses, including the highly pathogenic Zaire ebolavirus (ZEBOV), and allow study of the viral life cycle under biosafety level 2 conditions. However, current systems depend on the expression of viral helper nucleocapsid proteins in target cells, thus making it impossible to determine whether ribonucleoprotein complexes transferred by iVLPs are able to facilitate initial transcription, an indispensable step in natural infection. Here we describe a ZEBOV iVLP system which overcomes this limitation and show that VP24 is essential for the formation of a functional ribonucleoprotein complex.

Original languageEnglish (US)
Pages (from-to)7260-7264
Number of pages5
JournalJournal of Virology
Volume80
Issue number14
DOIs
StatePublished - Jul 1 2006
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Immunology

Cite this

Hoenen, T., Groseth, A., Kolesnikova, L., Theriault, S., Ebihara, H., Hartlieb, B., Bamberg, S., Feldmann, H., Ströher, U., & Becker, S. (2006). Infection of naïve target cells with virus-like particles: Implications for the function of ebola virus VP24. Journal of Virology, 80(14), 7260-7264. https://doi.org/10.1128/JVI.00051-06