Induction of renal growth and injury in the intact rat kidney by dietary deficiency of antioxidants

Karl A Nath, Abdulla K. Salahudeen, Anthony J. Croatt, Stefan M. Kren

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

We report induction of renal growth and injury in the intact rat kidney using a diet deficient in vitamin E and selenium. This diet was imposed in 3-wk-old male weanling rats, and after 9 wk, enhancement of growth, characterized by increased wet weight, dry weight, protein content, and DNA content appeared. Morphometric analyses revealed increased kidney volume, tubular epithelial volume, and mean glomerular volume. There were no differences in nephron number. The animals on the deficient diet displayed increased urinary protein excretion at 9 wk. Renal injury was also characterized by an interstitial cellular infiltrate and diminutions in glomerular filtration rate. Enhanced growth and injury were antedated by increased renal ammoniagenesis. The deficient diet did not induce metabolic acidosis, potassium depletion, glucose intolerance, or elevated plasma amino acid concentration. Enhancement of renal growth and ammoniagenesis by the deficient diet was not suppressible by chronic alkali therapy. Stimulation of renal growth could not be ascribed to increased intrarenal iron, induction of ornithine decarboxylase, or alterations in glomerular hemodynamics. Stimulation of renal ammoniagenesis by dietary deficiency of antioxidants is a novel finding, as is induction of growth and injury. We suggest that increased renal ammoniagenesis contributes to induction of renal growth and injury.

Original languageEnglish (US)
Pages (from-to)1179-1192
Number of pages14
JournalJournal of Clinical Investigation
Volume86
Issue number4
StatePublished - Oct 1990
Externally publishedYes

Fingerprint

Antioxidants
Kidney
Wounds and Injuries
Growth
Diet
Weights and Measures
Ornithine Decarboxylase
Glucose Intolerance
Nephrons
Alkalies
Selenium
Acidosis
Glomerular Filtration Rate
Vitamin E
Potassium
Proteins
Iron
Hemodynamics
Amino Acids
DNA

Keywords

  • Ammonia oxidants
  • Growth
  • Injury
  • Kidney

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Induction of renal growth and injury in the intact rat kidney by dietary deficiency of antioxidants. / Nath, Karl A; Salahudeen, Abdulla K.; Croatt, Anthony J.; Kren, Stefan M.

In: Journal of Clinical Investigation, Vol. 86, No. 4, 10.1990, p. 1179-1192.

Research output: Contribution to journalArticle

Nath, Karl A ; Salahudeen, Abdulla K. ; Croatt, Anthony J. ; Kren, Stefan M. / Induction of renal growth and injury in the intact rat kidney by dietary deficiency of antioxidants. In: Journal of Clinical Investigation. 1990 ; Vol. 86, No. 4. pp. 1179-1192.
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