Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice

Lu Zhuang, Xianbao Zhan, Yan Bi, Baoan D Ji

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Pancreatitis is a major risk factor for the development of pancreatic cancer. In genetically engineered mouse models, induction of pancreatic inflammation dramatically accelerates oncogenic KRas-induced fibrosis, precancerous PanIN formation, and tumorigenesis. Here we describe simple methods of secretagogue-induced experimental acute and chronic pancreatitis, the most commonly used pancreatitis models, and their applications in pancreatic cancer research. Additionally, the preparation of primary pancreatic acinar cells is introduced. Primary acinar cells can be used to study the early events of pancreatic inflammation and pancreatic acinar-to-ductal (ADM) metaplasia.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages287-297
Number of pages11
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Keywords

  • Acinar cell
  • Caerulein
  • CCK
  • KRas
  • Pancreatic cancer
  • Pancreatitis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Zhuang, L., Zhan, X., Bi, Y., & Ji, B. D. (2019). Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice. In Methods in Molecular Biology (pp. 287-297). (Methods in Molecular Biology; Vol. 1882). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8879-2_25