Abstract
Pancreatitis is a major risk factor for the development of pancreatic cancer. In genetically engineered mouse models, induction of pancreatic inflammation dramatically accelerates oncogenic KRas-induced fibrosis, precancerous PanIN formation, and tumorigenesis. Here we describe simple methods of secretagogue-induced experimental acute and chronic pancreatitis, the most commonly used pancreatitis models, and their applications in pancreatic cancer research. Additionally, the preparation of primary pancreatic acinar cells is introduced. Primary acinar cells can be used to study the early events of pancreatic inflammation and pancreatic acinar-to-ductal (ADM) metaplasia.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 287-297 |
| Number of pages | 11 |
| DOIs | |
| State | Published - Jan 1 2019 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1882 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Acinar cell
- Caerulein
- CCK
- KRas
- Pancreatic cancer
- Pancreatitis
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice. / Zhuang, Lu; Zhan, Xianbao; Bi, Yan; Ji, Baoan D.
Methods in Molecular Biology. Humana Press Inc., 2019. p. 287-297 (Methods in Molecular Biology; Vol. 1882).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice
AU - Zhuang, Lu
AU - Zhan, Xianbao
AU - Bi, Yan
AU - Ji, Baoan D
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Pancreatitis is a major risk factor for the development of pancreatic cancer. In genetically engineered mouse models, induction of pancreatic inflammation dramatically accelerates oncogenic KRas-induced fibrosis, precancerous PanIN formation, and tumorigenesis. Here we describe simple methods of secretagogue-induced experimental acute and chronic pancreatitis, the most commonly used pancreatitis models, and their applications in pancreatic cancer research. Additionally, the preparation of primary pancreatic acinar cells is introduced. Primary acinar cells can be used to study the early events of pancreatic inflammation and pancreatic acinar-to-ductal (ADM) metaplasia.
AB - Pancreatitis is a major risk factor for the development of pancreatic cancer. In genetically engineered mouse models, induction of pancreatic inflammation dramatically accelerates oncogenic KRas-induced fibrosis, precancerous PanIN formation, and tumorigenesis. Here we describe simple methods of secretagogue-induced experimental acute and chronic pancreatitis, the most commonly used pancreatitis models, and their applications in pancreatic cancer research. Additionally, the preparation of primary pancreatic acinar cells is introduced. Primary acinar cells can be used to study the early events of pancreatic inflammation and pancreatic acinar-to-ductal (ADM) metaplasia.
KW - Acinar cell
KW - Caerulein
KW - CCK
KW - KRas
KW - Pancreatic cancer
KW - Pancreatitis
UR - http://www.scopus.com/inward/record.url?scp=85055636494&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055636494&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8879-2_25
DO - 10.1007/978-1-4939-8879-2_25
M3 - Chapter
C2 - 30378063
AN - SCOPUS:85055636494
T3 - Methods in Molecular Biology
SP - 287
EP - 297
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -