Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice

Lu Zhuang, Xianbao Zhan, Yan Bi, Baoan D Ji

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Pancreatitis is a major risk factor for the development of pancreatic cancer. In genetically engineered mouse models, induction of pancreatic inflammation dramatically accelerates oncogenic KRas-induced fibrosis, precancerous PanIN formation, and tumorigenesis. Here we describe simple methods of secretagogue-induced experimental acute and chronic pancreatitis, the most commonly used pancreatitis models, and their applications in pancreatic cancer research. Additionally, the preparation of primary pancreatic acinar cells is introduced. Primary acinar cells can be used to study the early events of pancreatic inflammation and pancreatic acinar-to-ductal (ADM) metaplasia.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages287-297
Number of pages11
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Fingerprint

Acinar Cells
Pancreatic Neoplasms
Pancreatitis
Carcinogenesis
Inflammation
Chronic Pancreatitis
Metaplasia
Fibrosis
Research

Keywords

  • Acinar cell
  • Caerulein
  • CCK
  • KRas
  • Pancreatic cancer
  • Pancreatitis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zhuang, L., Zhan, X., Bi, Y., & Ji, B. D. (2019). Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice. In Methods in Molecular Biology (pp. 287-297). (Methods in Molecular Biology; Vol. 1882). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8879-2_25

Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice. / Zhuang, Lu; Zhan, Xianbao; Bi, Yan; Ji, Baoan D.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 287-297 (Methods in Molecular Biology; Vol. 1882).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Zhuang, L, Zhan, X, Bi, Y & Ji, BD 2019, Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1882, Humana Press Inc., pp. 287-297. https://doi.org/10.1007/978-1-4939-8879-2_25
Zhuang L, Zhan X, Bi Y, Ji BD. Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 287-297. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8879-2_25
Zhuang, Lu ; Zhan, Xianbao ; Bi, Yan ; Ji, Baoan D. / Induction of pancreatic inflammation accelerates pancreatic tumorigenesis in mice. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 287-297 (Methods in Molecular Biology).
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