Induction of myelin genes during peripheral nerve remyelination requires a continuous signal from the ingrowing axon

S. K. Gupta, J. Pringle, J. F. Poduslo, C. Mezei

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The effect of a permanent transection on myelin gene expression in a regenerating sciatic nerve and in an adult sciatic nerve was compared to establish the degree of axonal control exerted upon Schwann cells in each population. First, the adult sciatic nerve was crushed, and the distal segment allowed to regenerate. At 12 days post-crush, the sciatic nerve was transected distal to the site of crush to disrupt the Schwann cell-axonal contacts that had reformed. Messenger RNA (mRNA) levels coding for five myelin proteins were assayed in the distal segment of the crush-transected nerve after 9 days and were compared to corresponding levels in the distal segments of sciatic nerves at 21 days post-crush and 21 days post-transection using Northern blot and slot-blot analysis. Levels of mRNAs found in the distal segment of the transected and crush-transected nerve suggested that Schwann cells in the regenerating nerve and in the mature adult nerve are equally responsive to axonal influences. The crush-transected model allowed the genes that were studied to be classified according to their response to Schwann cell-axonal contact. The levels of mRNAs were 1) down-regulated to basal levels (PO and MBP mRNAs), 2) down-regulated to undetectable levels (myelin-associated glycoprotein mRNAs), 3) up-regulated (mRNAs encoding 2'3'- cyclic nucleotide phosphodiesterase and β-actin), or 4) not stringently controlled by the removal of Schwann cell-axonal contact (proteolipid protein mRNAs). This novel experimental model has thus provided evidence that the expression of some of the important myelin genes during peripheral nerve regeneration is dependent on continuous signals from the ingrowing axons.

Original languageEnglish (US)
Pages (from-to)14-23
Number of pages10
JournalJournal of Neuroscience Research
Volume34
Issue number1
DOIs
StatePublished - 1993

Fingerprint

Myelin Sheath
Peripheral Nerves
Axons
Schwann Cells
Sciatic Nerve
Messenger RNA
Genes
Nerve Crush
Myelin-Associated Glycoprotein
Proteolipids
Myelin Proteins
Nerve Regeneration
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Northern Blotting
Actins
Theoretical Models
Gene Expression
Population
Proteins

Keywords

  • gene expression
  • mRNA levels
  • regeneration
  • Schwann cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Induction of myelin genes during peripheral nerve remyelination requires a continuous signal from the ingrowing axon. / Gupta, S. K.; Pringle, J.; Poduslo, J. F.; Mezei, C.

In: Journal of Neuroscience Research, Vol. 34, No. 1, 1993, p. 14-23.

Research output: Contribution to journalArticle

Gupta, S. K. ; Pringle, J. ; Poduslo, J. F. ; Mezei, C. / Induction of myelin genes during peripheral nerve remyelination requires a continuous signal from the ingrowing axon. In: Journal of Neuroscience Research. 1993 ; Vol. 34, No. 1. pp. 14-23.
@article{19ec052d31614dd6b64596cdc3b15e6a,
title = "Induction of myelin genes during peripheral nerve remyelination requires a continuous signal from the ingrowing axon",
abstract = "The effect of a permanent transection on myelin gene expression in a regenerating sciatic nerve and in an adult sciatic nerve was compared to establish the degree of axonal control exerted upon Schwann cells in each population. First, the adult sciatic nerve was crushed, and the distal segment allowed to regenerate. At 12 days post-crush, the sciatic nerve was transected distal to the site of crush to disrupt the Schwann cell-axonal contacts that had reformed. Messenger RNA (mRNA) levels coding for five myelin proteins were assayed in the distal segment of the crush-transected nerve after 9 days and were compared to corresponding levels in the distal segments of sciatic nerves at 21 days post-crush and 21 days post-transection using Northern blot and slot-blot analysis. Levels of mRNAs found in the distal segment of the transected and crush-transected nerve suggested that Schwann cells in the regenerating nerve and in the mature adult nerve are equally responsive to axonal influences. The crush-transected model allowed the genes that were studied to be classified according to their response to Schwann cell-axonal contact. The levels of mRNAs were 1) down-regulated to basal levels (PO and MBP mRNAs), 2) down-regulated to undetectable levels (myelin-associated glycoprotein mRNAs), 3) up-regulated (mRNAs encoding 2'3'- cyclic nucleotide phosphodiesterase and β-actin), or 4) not stringently controlled by the removal of Schwann cell-axonal contact (proteolipid protein mRNAs). This novel experimental model has thus provided evidence that the expression of some of the important myelin genes during peripheral nerve regeneration is dependent on continuous signals from the ingrowing axons.",
keywords = "gene expression, mRNA levels, regeneration, Schwann cell",
author = "Gupta, {S. K.} and J. Pringle and Poduslo, {J. F.} and C. Mezei",
year = "1993",
doi = "10.1002/jnr.490340103",
language = "English (US)",
volume = "34",
pages = "14--23",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Induction of myelin genes during peripheral nerve remyelination requires a continuous signal from the ingrowing axon

AU - Gupta, S. K.

AU - Pringle, J.

AU - Poduslo, J. F.

AU - Mezei, C.

PY - 1993

Y1 - 1993

N2 - The effect of a permanent transection on myelin gene expression in a regenerating sciatic nerve and in an adult sciatic nerve was compared to establish the degree of axonal control exerted upon Schwann cells in each population. First, the adult sciatic nerve was crushed, and the distal segment allowed to regenerate. At 12 days post-crush, the sciatic nerve was transected distal to the site of crush to disrupt the Schwann cell-axonal contacts that had reformed. Messenger RNA (mRNA) levels coding for five myelin proteins were assayed in the distal segment of the crush-transected nerve after 9 days and were compared to corresponding levels in the distal segments of sciatic nerves at 21 days post-crush and 21 days post-transection using Northern blot and slot-blot analysis. Levels of mRNAs found in the distal segment of the transected and crush-transected nerve suggested that Schwann cells in the regenerating nerve and in the mature adult nerve are equally responsive to axonal influences. The crush-transected model allowed the genes that were studied to be classified according to their response to Schwann cell-axonal contact. The levels of mRNAs were 1) down-regulated to basal levels (PO and MBP mRNAs), 2) down-regulated to undetectable levels (myelin-associated glycoprotein mRNAs), 3) up-regulated (mRNAs encoding 2'3'- cyclic nucleotide phosphodiesterase and β-actin), or 4) not stringently controlled by the removal of Schwann cell-axonal contact (proteolipid protein mRNAs). This novel experimental model has thus provided evidence that the expression of some of the important myelin genes during peripheral nerve regeneration is dependent on continuous signals from the ingrowing axons.

AB - The effect of a permanent transection on myelin gene expression in a regenerating sciatic nerve and in an adult sciatic nerve was compared to establish the degree of axonal control exerted upon Schwann cells in each population. First, the adult sciatic nerve was crushed, and the distal segment allowed to regenerate. At 12 days post-crush, the sciatic nerve was transected distal to the site of crush to disrupt the Schwann cell-axonal contacts that had reformed. Messenger RNA (mRNA) levels coding for five myelin proteins were assayed in the distal segment of the crush-transected nerve after 9 days and were compared to corresponding levels in the distal segments of sciatic nerves at 21 days post-crush and 21 days post-transection using Northern blot and slot-blot analysis. Levels of mRNAs found in the distal segment of the transected and crush-transected nerve suggested that Schwann cells in the regenerating nerve and in the mature adult nerve are equally responsive to axonal influences. The crush-transected model allowed the genes that were studied to be classified according to their response to Schwann cell-axonal contact. The levels of mRNAs were 1) down-regulated to basal levels (PO and MBP mRNAs), 2) down-regulated to undetectable levels (myelin-associated glycoprotein mRNAs), 3) up-regulated (mRNAs encoding 2'3'- cyclic nucleotide phosphodiesterase and β-actin), or 4) not stringently controlled by the removal of Schwann cell-axonal contact (proteolipid protein mRNAs). This novel experimental model has thus provided evidence that the expression of some of the important myelin genes during peripheral nerve regeneration is dependent on continuous signals from the ingrowing axons.

KW - gene expression

KW - mRNA levels

KW - regeneration

KW - Schwann cell

UR - http://www.scopus.com/inward/record.url?scp=0027505381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027505381&partnerID=8YFLogxK

U2 - 10.1002/jnr.490340103

DO - 10.1002/jnr.490340103

M3 - Article

C2 - 7678657

AN - SCOPUS:0027505381

VL - 34

SP - 14

EP - 23

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 1

ER -