Induction of iNOS in human saphenous veins

D. G. Cable, Hartzell V Schaff

Research output: Contribution to journalArticle

Abstract

E.coli lipopolysacharide (LPS) stimulates inducible nitric oxide synthase (iNOS) in animal vascular smooth muscle but iNOS has not been documented in human saphenous veins. Previous investigators failed to demonstrate iNOS in human saphenous veins with LPS stimulation. We exposed human saphenous veins to E. coli LPS (100μgm/ml) for 20hr and suspended in organ chambers. Veins were contracted with a maximum dose of KCl (80mM). There was no difference in KCl contraction in either controls or LPS veins (1.0±0.2gm vs 1.7±0.7gm, n=5, t-test). Veins were then contracted with norepinephrine (NE, 10-9-10-5M). LPS veins had a reduced contraction to NE, both developed tension and KCl-normalized tension were reduced compared to controls (n=5, P<0.05, ANOVA). Indomethacin (10-5M) pretreatment did not alter the reduced NE contraction in LPS veins (n=5), but L-NMMA (10-4M) pretreatment attenuated the contraction differential so LPS vessels exposed to NE contracted similar to controls. Tetraethylammonium chloride (TEA, 10-3M) pretreatment likewise reversed the contraction differential (n=5), demonstrating reversal of nitric oxide hyperpolarization. This study demonstrates induction of iNOS in human saphenous veins for the first time, contradicting previous investigations.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

saphenous vein
Saphenous Vein
Nitric Oxide Synthase Type II
Veins
pretreatment
Escherichia coli
indomethacin
omega-N-Methylarginine
norepinephrine
blood vessels
Tetraethylammonium
smooth muscle
nitric oxide
chlorides
Analysis of variance (ANOVA)
Vascular Smooth Muscle
Indomethacin
analysis of variance
Muscle
Norepinephrine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Induction of iNOS in human saphenous veins. / Cable, D. G.; Schaff, Hartzell V.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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AB - E.coli lipopolysacharide (LPS) stimulates inducible nitric oxide synthase (iNOS) in animal vascular smooth muscle but iNOS has not been documented in human saphenous veins. Previous investigators failed to demonstrate iNOS in human saphenous veins with LPS stimulation. We exposed human saphenous veins to E. coli LPS (100μgm/ml) for 20hr and suspended in organ chambers. Veins were contracted with a maximum dose of KCl (80mM). There was no difference in KCl contraction in either controls or LPS veins (1.0±0.2gm vs 1.7±0.7gm, n=5, t-test). Veins were then contracted with norepinephrine (NE, 10-9-10-5M). LPS veins had a reduced contraction to NE, both developed tension and KCl-normalized tension were reduced compared to controls (n=5, P<0.05, ANOVA). Indomethacin (10-5M) pretreatment did not alter the reduced NE contraction in LPS veins (n=5), but L-NMMA (10-4M) pretreatment attenuated the contraction differential so LPS vessels exposed to NE contracted similar to controls. Tetraethylammonium chloride (TEA, 10-3M) pretreatment likewise reversed the contraction differential (n=5), demonstrating reversal of nitric oxide hyperpolarization. This study demonstrates induction of iNOS in human saphenous veins for the first time, contradicting previous investigations.

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