TY - JOUR
T1 - Induction of human IgE synthesis in B cells by mast cells and basophils
AU - Gauchat, Jean François
AU - Henchoz, Sybille
AU - Mazzei, Gonzalo
AU - Aubry, Jean Pierre
AU - Brunner, Thomas
AU - Blasey, Horst
AU - Life, Paul
AU - Talabot, Dominique
AU - Flores-Romo, Leopoldo
AU - Thompson, Jeff
AU - Kishi, Kenji
AU - Butterfield, Joseph
AU - Dahinden, Clemens
AU - Bonnefoy, Jean Yves
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1993
Y1 - 1993
N2 - IMMUNOGLOBULIN E (IgE) is central to the induction of allergic diseases through its binding to the high-affinity receptor (FcεRl) on mast cells and basophils. Crosslinking by allergens of the bound IgE leads to the release of various inflammatory mediators. IgE production by B cells requires a physical interaction with T cells1, involving a number of surface adhesion molecules1,2, as well as the soluble factors interleukin-4 (IL-4)3,4 and IL-13 (ref. 5) produced by T cells6,7, basophils8 and mast cells9. Here we report that, in the presence of IL-4, mast and basophilic cell lines can provide the cell contact signals that are required for IgE synthesis. The human cell lines HMC-1 (mast) and KU812 (basophilic) both express the ligand for CD40 (CD40L) which is shown to be responsible for the IgE production. Moreover, freshly isolated purified human lung mast cells and blood basophils are also shown to express CD40L and to induce IgE production. This evidence suggests that mast cells and basophils may therefore play a key role in allergy not only by producing inflammatory mediators, but also by directly regulating IgE production independently of T cells.
AB - IMMUNOGLOBULIN E (IgE) is central to the induction of allergic diseases through its binding to the high-affinity receptor (FcεRl) on mast cells and basophils. Crosslinking by allergens of the bound IgE leads to the release of various inflammatory mediators. IgE production by B cells requires a physical interaction with T cells1, involving a number of surface adhesion molecules1,2, as well as the soluble factors interleukin-4 (IL-4)3,4 and IL-13 (ref. 5) produced by T cells6,7, basophils8 and mast cells9. Here we report that, in the presence of IL-4, mast and basophilic cell lines can provide the cell contact signals that are required for IgE synthesis. The human cell lines HMC-1 (mast) and KU812 (basophilic) both express the ligand for CD40 (CD40L) which is shown to be responsible for the IgE production. Moreover, freshly isolated purified human lung mast cells and blood basophils are also shown to express CD40L and to induce IgE production. This evidence suggests that mast cells and basophils may therefore play a key role in allergy not only by producing inflammatory mediators, but also by directly regulating IgE production independently of T cells.
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U2 - 10.1038/365340a0
DO - 10.1038/365340a0
M3 - Article
C2 - 7690905
AN - SCOPUS:0027427165
SN - 0028-0836
VL - 365
SP - 340
EP - 343
JO - Nature
JF - Nature
IS - 6444
ER -