Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor β: A proposed model for indirect mitogenesis involving autocrine activity

E. B. Leof, J. A. Proper, A. S. Goustin, G. D. Shipley, P. E. DiCorleto, H. L. Moses

Research output: Contribution to journalArticlepeer-review

387 Scopus citations

Abstract

Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth factor β resulted in an early induction of c-sis mRNA. The increase in c-sis mRNA was followed by a corresponding increase in protein similar to platelet-derived growth factor (PDGF) in the culture medium. In addition, PDGF-regulated genes (c-fos and c-myc) were stimulated by transforming growth factor β with delayed kinetics relative to that seen in other cell systems with direct PDGF stimulation. A model is proposed in which the monolayer mitogenicity of transforming growth factor β is mediated by the induction of c-sis and PDGF and the subsequent autocrine stimulation of c-fos, c-myc, and other PDGF-inducible genes.

Original languageEnglish (US)
Pages (from-to)2453-2457
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number8
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • General

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