Abstract
Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth factor β resulted in an early induction of c-sis mRNA. The increase in c-sis mRNA was followed by a corresponding increase in protein similar to platelet-derived growth factor (PDGF) in the culture medium. In addition, PDGF-regulated genes (c-fos and c-myc) were stimulated by transforming growth factor β with delayed kinetics relative to that seen in other cell systems with direct PDGF stimulation. A model is proposed in which the monolayer mitogenicity of transforming growth factor β is mediated by the induction of c-sis and PDGF and the subsequent autocrine stimulation of c-fos, c-myc, and other PDGF-inducible genes.
Original language | English (US) |
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Pages (from-to) | 2453-2457 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 83 |
Issue number | 8 |
DOIs | |
State | Published - 1986 |
ASJC Scopus subject areas
- General