Induction of angiogenesis in the β-amyloid peptide-injected rat hippocampus

Ladan Zand, Jae K. Ryu, James G. McLarnon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Angiogenesis, the formation of new capillary blood vessels, has been studied following the stereotaxic injection of β-amyloid peptide (Aβ1-42) into rat hippocampus. Immunohistochemical analysis for laminin showed that neovascularization was only slightly increased, relative to control, in the hippocampus 1 day post-Aβ1-42 injection. However, 7 days following peptide injection neovascularization was markedly up-regulated (by 2.2-fold) compared to control. Immunoreactivity for the angiogenic stimulatory agent vascular endothelial growth factor (VEGF) was also significantly increased in the hippocampus 7 days after Aβ1-42 injection. Double immunofluorescence staining demonstrated that the increased level of VEGF immunoreactivity was localized to both astrocytes and microglia, suggesting inflammatory responses contributed to angiogenesis. The findings of β-amyloid stimulated angiogenesis and the involvement of peptide-induced inflammatory processes may have relevance to the pathology of Alzheimer's disease.

Original languageEnglish (US)
Pages (from-to)129-132
Number of pages4
JournalNeuroReport
Volume16
Issue number2
DOIs
StatePublished - Feb 8 2005
Externally publishedYes

Fingerprint

Amyloid
Hippocampus
Peptides
Injections
Vascular Endothelial Growth Factor A
Microglia
Laminin
Astrocytes
Fluorescent Antibody Technique
Blood Vessels
Alzheimer Disease
Pathology
Staining and Labeling

Keywords

  • Alzheimer's disease β-Amyloid peptide
  • Angiogenesis
  • Astrocytes
  • Inflammation
  • Microglia
  • VEGF

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Induction of angiogenesis in the β-amyloid peptide-injected rat hippocampus. / Zand, Ladan; Ryu, Jae K.; McLarnon, James G.

In: NeuroReport, Vol. 16, No. 2, 08.02.2005, p. 129-132.

Research output: Contribution to journalArticle

Zand, Ladan ; Ryu, Jae K. ; McLarnon, James G. / Induction of angiogenesis in the β-amyloid peptide-injected rat hippocampus. In: NeuroReport. 2005 ; Vol. 16, No. 2. pp. 129-132.
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