Induction of airway allergic inflammation by hypothiocyanite via epithelial cells

Shoichi Suzuki, Masahiro Ogawa, Shoichiro Ohta, Satoshi Nunomura, Yasuhiro Nanri, Hiroshi Shiraishi, Yasutaka Mitamura, Tomohito Yoshihara, James J. Lee, Kenji Izuhara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Hypothiocyanite (OSCN-) serves as a potent innate defense system against microbes in the lungs. OSCN- is generated by the catalysis of peroxidases using thiocyanate transported via several anion transporters, including pendrin/SLC26A4 and hydrogen peroxide (H2O2) generated by Duox1 and Duox2. We previously demonstrated that expression of pendrin, peroxidases, and Duox1/Duox2 is up-regulated in bronchial asthma patients and/or asthma model mice and that these molecules are important in accelerating airway inflammation. However, it remained unclear how activating these molecules would lead to airway inflammation. In this study, we examined whether OSCN- produced via the pendrin/peroxidase/Duox pathway causes inflammation via airway epithelial cells. In an in vitro OSCN- production system, OSCN-, but not H2O2, activated NF-κB, a transcription factor critical for inflammatory responses, in the airway epithelial cells. OSCN- was sensed by protein kinase A (PKA) followed by formation of the dimerization of PKA. The dimerized PKA, the active form, was critical in activating NF-κB. Detoxifying H2O2, mainly by catalase, enabled the dominant abilities of OSCN- to dimerize PKA and activate NF-κB, compared with untreated H2O2. Furthermore, OSCN- in high doses caused necrosis of the cells, inducing release of IL-33, a trigger to initiate type 2 inflammation. These results demonstrate that OSCN- in low doses activates NF-κB via PKA in airway epithelial cells, whereas OSCN- in high doses causes necrosis, suggesting an important role in airway allergic inflammation for the production of OSCN- via the pendrin/peroxidase/Duox pathway.

Original languageEnglish (US)
Pages (from-to)27219-27227
Number of pages9
JournalJournal of Biological Chemistry
Volume291
Issue number53
DOIs
StatePublished - Dec 30 2016

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Epithelial Cells
Inflammation
Cyclic AMP-Dependent Protein Kinases
Peroxidases
Peroxidase
hypothiocyanite ion
Necrosis
Asthma
Molecules
Dimerization
Catalysis
Catalase
Hydrogen Peroxide
Anions
Transcription Factors
Lung

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Suzuki, S., Ogawa, M., Ohta, S., Nunomura, S., Nanri, Y., Shiraishi, H., ... Izuhara, K. (2016). Induction of airway allergic inflammation by hypothiocyanite via epithelial cells. Journal of Biological Chemistry, 291(53), 27219-27227. https://doi.org/10.1074/jbc.M116.746909

Induction of airway allergic inflammation by hypothiocyanite via epithelial cells. / Suzuki, Shoichi; Ogawa, Masahiro; Ohta, Shoichiro; Nunomura, Satoshi; Nanri, Yasuhiro; Shiraishi, Hiroshi; Mitamura, Yasutaka; Yoshihara, Tomohito; Lee, James J.; Izuhara, Kenji.

In: Journal of Biological Chemistry, Vol. 291, No. 53, 30.12.2016, p. 27219-27227.

Research output: Contribution to journalArticle

Suzuki, S, Ogawa, M, Ohta, S, Nunomura, S, Nanri, Y, Shiraishi, H, Mitamura, Y, Yoshihara, T, Lee, JJ & Izuhara, K 2016, 'Induction of airway allergic inflammation by hypothiocyanite via epithelial cells', Journal of Biological Chemistry, vol. 291, no. 53, pp. 27219-27227. https://doi.org/10.1074/jbc.M116.746909
Suzuki S, Ogawa M, Ohta S, Nunomura S, Nanri Y, Shiraishi H et al. Induction of airway allergic inflammation by hypothiocyanite via epithelial cells. Journal of Biological Chemistry. 2016 Dec 30;291(53):27219-27227. https://doi.org/10.1074/jbc.M116.746909
Suzuki, Shoichi ; Ogawa, Masahiro ; Ohta, Shoichiro ; Nunomura, Satoshi ; Nanri, Yasuhiro ; Shiraishi, Hiroshi ; Mitamura, Yasutaka ; Yoshihara, Tomohito ; Lee, James J. ; Izuhara, Kenji. / Induction of airway allergic inflammation by hypothiocyanite via epithelial cells. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 53. pp. 27219-27227.
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abstract = "Hypothiocyanite (OSCN-) serves as a potent innate defense system against microbes in the lungs. OSCN- is generated by the catalysis of peroxidases using thiocyanate transported via several anion transporters, including pendrin/SLC26A4 and hydrogen peroxide (H2O2) generated by Duox1 and Duox2. We previously demonstrated that expression of pendrin, peroxidases, and Duox1/Duox2 is up-regulated in bronchial asthma patients and/or asthma model mice and that these molecules are important in accelerating airway inflammation. However, it remained unclear how activating these molecules would lead to airway inflammation. In this study, we examined whether OSCN- produced via the pendrin/peroxidase/Duox pathway causes inflammation via airway epithelial cells. In an in vitro OSCN- production system, OSCN-, but not H2O2, activated NF-κB, a transcription factor critical for inflammatory responses, in the airway epithelial cells. OSCN- was sensed by protein kinase A (PKA) followed by formation of the dimerization of PKA. The dimerized PKA, the active form, was critical in activating NF-κB. Detoxifying H2O2, mainly by catalase, enabled the dominant abilities of OSCN- to dimerize PKA and activate NF-κB, compared with untreated H2O2. Furthermore, OSCN- in high doses caused necrosis of the cells, inducing release of IL-33, a trigger to initiate type 2 inflammation. These results demonstrate that OSCN- in low doses activates NF-κB via PKA in airway epithelial cells, whereas OSCN- in high doses causes necrosis, suggesting an important role in airway allergic inflammation for the production of OSCN- via the pendrin/peroxidase/Duox pathway.",
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