Induction- and conditioning-protocol dependent involvement of NR2B-containing NMDA receptors in synaptic potentiation and contextual fear memory in the hippocampal CA1 region of rats.

Xue Han Zhang, LongJun Wu, B. Gong, Ming Ren, Bao Ming Li, Min Zhuo

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Long-term potentiation (LTP) in the hippocampal CA1 region requires the activation of N-methyl-D-aspartate receptors (NMDARs). Studies using genetic and pharmacological approaches have reported inconsistent results of the requirement of NR2B-containing NMDARs in LTP in the CA1 region. Pharmacological studies showed that NR2B-containing NMDARs are not required for LTP, while genetic studies reported that over-expression of NR2B-NMDARs enhances LTP and hippocampus-dependent memory. Here, we provide evidence showing that the functional role of NR2B-NMDARs in hippocampal LTP and memory depends on LTP-inducing and behavior-conditioning protocols. Inhibition of NR2B-NMDARs with the NR2B selective antagonist ifenprodil or Ro25-6981 suppressed LTP induced by spike-timing protocol, with no impact on LTP induced by pairing protocol or two-train high-frequency stimulation (HFS) protocol. Inhibition of NR2B-NMDARs did not affect the late phase LTP induced by four-train HFS. Ca2(+) imaging showed that there was difference in kinetics of intracellular Ca2(+) signals induced by spiking-timing and pairing protocols. Pre-training intra-CA1 infusion of ifenprodil or Ro25-6981 impaired the contextual fear memory induced by five CS-US pairings, with no effect on the memory induced by one CS-US pairing.

Original languageEnglish (US)
Number of pages1
JournalMolecular Brain
Volume1
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

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Hippocampal CA1 Region
Long-Term Potentiation
Fear
Pharmacology
NR2B NMDA receptor
Conditioning (Psychology)
Long-Term Memory
N-Methyl-D-Aspartate Receptors
Hippocampus

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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Induction- and conditioning-protocol dependent involvement of NR2B-containing NMDA receptors in synaptic potentiation and contextual fear memory in the hippocampal CA1 region of rats. / Zhang, Xue Han; Wu, LongJun; Gong, B.; Ren, Ming; Li, Bao Ming; Zhuo, Min.

In: Molecular Brain, Vol. 1, 01.01.2008.

Research output: Contribution to journalArticle

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