Indiscriminate loss of myenteric neurones in the TNBS-inflamed guinea-pig distal colon

David R Linden, J. M. Couvrette, A. Ciolino, C. McQuoid, H. Blaszyk, K. A. Sharkey, Gary M. Mawe

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

This investigation was conducted to establish whether guinea-pig trinitrobenzene sulfonic acid (TNBS)-colitis was associated with a change in the number of neurones of the myenteric plexus, and, if so, whether select subpopulations of neurones were affected. Total neurones were quantified with human (Hu) antiserum, and subpopulations were evaluated with antisera directed against choline acetyltransferase, nitric oxide synthase, calretinin, neuronal nuclear protein or vasoactive intestinal peptide (VIP). Colitis was associated with a loss of 20% of the myenteric neurones, most of which occurred during the first 12 h past-TNBS administration. During this period, myenteric ganglia were infiltrated with neutrophils while lymphocytes appeared at a later time-point. The neuronal loss persisted at a 56-day time-point, when inflammation had resolved. The decrease in myenteric neurones was not associated with a decrease in any given subpopulation of neurones, but the proportion of VIP-immunoreactive neurones increased 6 days following TNBS administration and returned to the control range at the 56 days. These findings indicate that there is an indiscriminant loss of myenteric neurones that occurs during the onset of TNBS-colitis, and the loss of neurones may be associated with the appearance of neutrophils in the region.

Original languageEnglish (US)
Pages (from-to)751-760
Number of pages10
JournalNeurogastroenterology and Motility
Volume17
Issue number5
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Trinitrobenzenes
Sulfonic Acids
Guinea Pigs
Colon
Neurons
Colitis
Vasoactive Intestinal Peptide
Immune Sera
Neutrophils
Calbindin 2
Myenteric Plexus
Nitric Oxide Synthase Type I
Choline O-Acetyltransferase
Nuclear Proteins
Ganglia
Lymphocytes
Inflammation

Keywords

  • Colitis
  • Enteric nervous system
  • Inflammatory bowel disease
  • Neutrophils

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Neuroscience(all)

Cite this

Indiscriminate loss of myenteric neurones in the TNBS-inflamed guinea-pig distal colon. / Linden, David R; Couvrette, J. M.; Ciolino, A.; McQuoid, C.; Blaszyk, H.; Sharkey, K. A.; Mawe, Gary M.

In: Neurogastroenterology and Motility, Vol. 17, No. 5, 10.2005, p. 751-760.

Research output: Contribution to journalArticle

Linden, David R ; Couvrette, J. M. ; Ciolino, A. ; McQuoid, C. ; Blaszyk, H. ; Sharkey, K. A. ; Mawe, Gary M. / Indiscriminate loss of myenteric neurones in the TNBS-inflamed guinea-pig distal colon. In: Neurogastroenterology and Motility. 2005 ; Vol. 17, No. 5. pp. 751-760.
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