Increasing vimentin expression associated with differentiation of human and rat preadipocytes

K. Teichert-Kuliszewska, B. S. Hamilton, Dak Roncari, James L Kirkland, W. S. Gillon, M. Deitel, C. H. Hollenberg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Alterations in the cytoskeletal apparatus constitute some of the earliest changes during assumption of an adipogenic phenotype. We examined three major cytoskeletal elements, β-actin, α-tubulin and vimentin, during adipogenesis in euploid cells from human and rat adipose tissue. As reported with 3T3 sub-lines, mRNA levels for β-actin and α-tubulin were decreased upon differentiation. However, in contrast to reports with 3T3 cells, levels of vimentin were increased during differentiation. Furthermore, immunological analyses confirmed that there was no decrease in vimentin protein levels during adipogenic development. As well as highlighting a difference between 3T3 cell lines and preadipocytes isolated from fat depots, these studies indicate that the pattern of cytoskeletal gene expression undergoes complex changes early during preadipocyte differentiation.

Original languageEnglish (US)
JournalInternational Journal of Obesity
Volume20
Issue numberSUPPL. 3
StatePublished - 1996
Externally publishedYes

Fingerprint

vimentin
Vimentin
3T3 Cells
Tubulin
tubulin
actin
Actins
rats
Adipogenesis
adipose tissue
Adipose Tissue
Fats
cell lines
cells
Phenotype
Gene Expression
phenotype
Cell Line
gene expression
Messenger RNA

Keywords

  • α-tubulin
  • β-actin
  • Adipocytes
  • Differentiation
  • Obesity
  • Preadipocytes
  • Vimentin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Teichert-Kuliszewska, K., Hamilton, B. S., Roncari, D., Kirkland, J. L., Gillon, W. S., Deitel, M., & Hollenberg, C. H. (1996). Increasing vimentin expression associated with differentiation of human and rat preadipocytes. International Journal of Obesity, 20(SUPPL. 3).

Increasing vimentin expression associated with differentiation of human and rat preadipocytes. / Teichert-Kuliszewska, K.; Hamilton, B. S.; Roncari, Dak; Kirkland, James L; Gillon, W. S.; Deitel, M.; Hollenberg, C. H.

In: International Journal of Obesity, Vol. 20, No. SUPPL. 3, 1996.

Research output: Contribution to journalArticle

Teichert-Kuliszewska, K, Hamilton, BS, Roncari, D, Kirkland, JL, Gillon, WS, Deitel, M & Hollenberg, CH 1996, 'Increasing vimentin expression associated with differentiation of human and rat preadipocytes', International Journal of Obesity, vol. 20, no. SUPPL. 3.
Teichert-Kuliszewska K, Hamilton BS, Roncari D, Kirkland JL, Gillon WS, Deitel M et al. Increasing vimentin expression associated with differentiation of human and rat preadipocytes. International Journal of Obesity. 1996;20(SUPPL. 3).
Teichert-Kuliszewska, K. ; Hamilton, B. S. ; Roncari, Dak ; Kirkland, James L ; Gillon, W. S. ; Deitel, M. ; Hollenberg, C. H. / Increasing vimentin expression associated with differentiation of human and rat preadipocytes. In: International Journal of Obesity. 1996 ; Vol. 20, No. SUPPL. 3.
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