Increases in cyclic AMP potentiate competence formation in BALB/c‐3T3 cells

Walker Wharton, Edward B. Leof, Nancy Olashaw, H. S. Earp, W. J. Pledger

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The ability of platelet‐derived growth factor and fibroblast growth factor to stimulate the initiation of DNA synthesis in quiescent BALB/c‐3T3 cells was enhanced by cholera toxin. However, the addition of cholera toxin to unsupplemented medium was not mitogenic, nor did cholera toxin increase the mitogenic potential of mediuum supplemented with platelet‐poor plasma. The enhancement of serum‐induced DNA synthesis by cholera toxin was due to a specific effect on competence formation and not plasma‐controlled progression. Cholera toxin increased the rate of competence formation during a transient exposure of quiescent cells to platelet‐derived growth factor; this rate was further increased by the addition of isobutylmethylxanthine, a cyclic nucleotide phosphodiesterase inhibitor. Intracellular cyclic AMP concentrations in quiescent BALB/c‐3T3 cells were increased 2‐ to 3‐fold after the addition of cholera toxin. The addition of cholera toxin plus 30 m̈M isobutylmethylxanthine caused an even greater (7‐ to 8‐fold) increase in the cellular levels of cyclic AMP. That these increases in cyclic AMP concentrations mediated at least part of the increased sensitivity of quiescent cells to competence factors was substantiated by the observation that 0.01 to 1 mM monobutrylcyclic AMP or 8‐bromocyclic AMP also caused a concentration‐dependent potentiation of competence formation in quiescent cells during a transient exposure to platelet‐derived growth factor.

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalJournal of Cellular Physiology
Volume111
Issue number2
DOIs
StatePublished - May 1982

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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