Increased thymic output in HIV-negative patients after antiretroviral therapy

Daniel B. Graham, Michael P. Bell, Catherine J. Huntoon, Joel G R Weaver, Nanci Hawley, Andrew David Badley, David J. McKean

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To determine the effects of antiretroviral therapy on thymic output independent of HIV infection. Methods: Thymic output was evaluated by quantifying signal joint T-cell receptor (TCR) recombination excision circles in peripheral blood lymphocytes from HIV-negative patients undergoing prophylactic antiretroviral therapy. Additionally, effects of the HIV protease inhibitor nelfinavir were assessed in vivo on TCR-induced death of murine double-positive thymocytes. Results: Five out of seven HIV-negative patients undergoing prophylactic antiretroviral therapy exhibited a dramatic increase (1-3 log 10) in recent thymic emigrants containing signal joint TCR recombination excision circles while their peripheral T cell compartments remained relatively unaffected. None of the patients developed subsequent HIV infections. Interestingly, nelfinavir did not have significant effects on TCR-induced apoptosis of murine thymocytes in vivo. Conclusion: Antiretroviral therapy augments thymic output independent of HIV. Furthermore, nelfinavir does not dramatically affect TCR-induced thymocyte death in mice, thus central tolerance remains intact.

Original languageEnglish (US)
Pages (from-to)1467-1472
Number of pages6
JournalAIDS
Volume19
Issue number14
StatePublished - Sep 23 2005

Fingerprint

T-Cell Antigen Receptor
Nelfinavir
HIV
Thymocytes
Genetic Recombination
HIV Infections
Joints
Central Tolerance
HIV Protease Inhibitors
Therapeutics
Lymphocytes
Apoptosis
T-Lymphocytes

Keywords

  • Antiretroviral therapy
  • Apoptosis
  • Protease inhibitors
  • Recent thymic emigrants
  • sjTRECS
  • Thymocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Graham, D. B., Bell, M. P., Huntoon, C. J., Weaver, J. G. R., Hawley, N., Badley, A. D., & McKean, D. J. (2005). Increased thymic output in HIV-negative patients after antiretroviral therapy. AIDS, 19(14), 1467-1472.

Increased thymic output in HIV-negative patients after antiretroviral therapy. / Graham, Daniel B.; Bell, Michael P.; Huntoon, Catherine J.; Weaver, Joel G R; Hawley, Nanci; Badley, Andrew David; McKean, David J.

In: AIDS, Vol. 19, No. 14, 23.09.2005, p. 1467-1472.

Research output: Contribution to journalArticle

Graham, DB, Bell, MP, Huntoon, CJ, Weaver, JGR, Hawley, N, Badley, AD & McKean, DJ 2005, 'Increased thymic output in HIV-negative patients after antiretroviral therapy', AIDS, vol. 19, no. 14, pp. 1467-1472.
Graham DB, Bell MP, Huntoon CJ, Weaver JGR, Hawley N, Badley AD et al. Increased thymic output in HIV-negative patients after antiretroviral therapy. AIDS. 2005 Sep 23;19(14):1467-1472.
Graham, Daniel B. ; Bell, Michael P. ; Huntoon, Catherine J. ; Weaver, Joel G R ; Hawley, Nanci ; Badley, Andrew David ; McKean, David J. / Increased thymic output in HIV-negative patients after antiretroviral therapy. In: AIDS. 2005 ; Vol. 19, No. 14. pp. 1467-1472.
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