Increased production of nitric oxide in coronary arteries during congestive heart failure

Brian O'Murchu, Virginia M Miller, Mark A. Perrella, John C Jr. Burnett

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Experiments were designed to determine whether a heterogeneity of endothelium-dependent relaxations in arteries from different vascular beds exists in experimental congestive heart failure (CHF) and to determine the mediators of those responses. CHF was produced in dogs by rapid ventricular pacing for 15 d. Rings of coronary, femoral, and renal arteries with and without endothelium from control and CHF dogs were suspended in organ chambers for measurement of isometric force. In arteries contracted with prostaglandin F2 alpha, endothelium-dependent relaxations to BHT 920 (an alpha2-adrenergic agonist) were increased in coronary arteries from dogs with CHF (maximal relaxation: control -15±9% vs CHF -92±5%; n = 5-6; P < 0.05), with a modest enhancement in renal arteries. Relaxations to adenosine diphosphate and the calcium ionophore were unchanged. Relaxations to BHT 920 in CHF were reduced by N(G)-monomethyl-L-arginine (L-NMMA) and pertussis toxin but not by indomethacin. These data suggest that endothelium-dependent relaxations are affected heterogeneously in CHF. The enhanced response to alpha2-adrenergic agonists in the coronary artery is mediated by nitric oxide through a mechanism sensitive to inhibition by pertussis toxin. This selective increase in endothelium-dependent relaxations in the coronary artery may contribute to preserving coronary blood flow during CHF.

Original languageEnglish (US)
Pages (from-to)165-171
Number of pages7
JournalJournal of Clinical Investigation
Volume93
Issue number1
StatePublished - Jan 1994

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Coronary Vessels
Nitric Oxide
Heart Failure
Endothelium
Adrenergic alpha-2 Receptor Agonists
Pertussis Toxin
Dogs
Renal Artery
Arteries
omega-N-Methylarginine
Dinoprost
Calcium Ionophores
Femoral Artery
Indomethacin
Adenosine Diphosphate
Blood Vessels
Arginine

Keywords

  • adrenergic function
  • coronary blood flow
  • endothelium
  • guanine nucleotide-binding regulatory proteins
  • vasodilatation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Increased production of nitric oxide in coronary arteries during congestive heart failure. / O'Murchu, Brian; Miller, Virginia M; Perrella, Mark A.; Burnett, John C Jr.

In: Journal of Clinical Investigation, Vol. 93, No. 1, 01.1994, p. 165-171.

Research output: Contribution to journalArticle

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