Increased number of cardiomyocytes in cross-sections from tachycardia-induced cardiomyopathic hearts

Sofija Jovanovic, Aaron J. Grantham, James E. Tarara, John C Jr. Burnett, Aleksandarjovanovic, Andre Terzic

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Based on positive identification of DNA replication and mitotic division in cardiomyocytes isolated from failing hearts, it has been proposed that adult ventricular cardiomyocytes can gain the capacity to proliferate with progression of heart failure. However, due to the lack of a reliable method to distinctly image individual cardiac cells within the myocardial syntitium, such a concept still remains largely controversial. In.the present study, we used laser confocal microscopy, to image cross-sections of intact myocardium stained with fluorescein-conjugated wheat germ agglutinin and propidium iodide. This approach allowed to clearly separate the profile of individual myocytcs within cardiac tissue sections. We found that in the left ventricles of dogs, subjected to tachycardiainduced cardiomyopathy, the number of cells was significantly increased in both longitudinal and transversal sections. Treatment with the angiotensin-converting enzyme inhibitor, enalapril, reversed these changes to values similar to those found in controls. Therefore, this study provides evidence, at the HI situ level, for cellular hyperplasia in heart failure. This supports the more general notion that adult cardiomyocytes may not be terminally differentiated, and that an increase in cell number could contribute to the increase in left ventricular mass observed with progression of disease.

Original languageEnglish (US)
Pages (from-to)153-155
Number of pages3
JournalInternational Journal of Molecular Medicine
Volume3
Issue number2
StatePublished - 1999

Fingerprint

Cardiac Myocytes
Tachycardia
Confocal Microscopy
Heart Failure
Cell Count
Wheat Germ Agglutinins
Enalapril
Propidium
Fluorescein
DNA Replication
Cardiomyopathies
Angiotensin-Converting Enzyme Inhibitors
Heart Ventricles
Hyperplasia
Disease Progression
Myocardium
Dogs

Keywords

  • Angiotensinconverting enzyme
  • Enalapril
  • Fluorescein
  • Heart failure
  • Hyperplasia
  • Laser confocal microscopy
  • Morphometry
  • Pacing

ASJC Scopus subject areas

  • Genetics

Cite this

Increased number of cardiomyocytes in cross-sections from tachycardia-induced cardiomyopathic hearts. / Jovanovic, Sofija; Grantham, Aaron J.; Tarara, James E.; Burnett, John C Jr.; Aleksandarjovanovic, ; Terzic, Andre.

In: International Journal of Molecular Medicine, Vol. 3, No. 2, 1999, p. 153-155.

Research output: Contribution to journalArticle

Jovanovic, Sofija ; Grantham, Aaron J. ; Tarara, James E. ; Burnett, John C Jr. ; Aleksandarjovanovic, ; Terzic, Andre. / Increased number of cardiomyocytes in cross-sections from tachycardia-induced cardiomyopathic hearts. In: International Journal of Molecular Medicine. 1999 ; Vol. 3, No. 2. pp. 153-155.
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