TY - JOUR
T1 - Increased number of cardiomyocytes in cross-sections from tachycardia-induced cardiomyopathic hearts
AU - Jovanovic, Sofija
AU - Grantham, Aaron J.
AU - Tarara, James E.
AU - Burnett, John C.
AU - Aleksandarjovanovic,
AU - Terzic, Andre
PY - 1999
Y1 - 1999
N2 - Based on positive identification of DNA replication and mitotic division in cardiomyocytes isolated from failing hearts, it has been proposed that adult ventricular cardiomyocytes can gain the capacity to proliferate with progression of heart failure. However, due to the lack of a reliable method to distinctly image individual cardiac cells within the myocardial syntitium, such a concept still remains largely controversial. In.the present study, we used laser confocal microscopy, to image cross-sections of intact myocardium stained with fluorescein-conjugated wheat germ agglutinin and propidium iodide. This approach allowed to clearly separate the profile of individual myocytcs within cardiac tissue sections. We found that in the left ventricles of dogs, subjected to tachycardiainduced cardiomyopathy, the number of cells was significantly increased in both longitudinal and transversal sections. Treatment with the angiotensin-converting enzyme inhibitor, enalapril, reversed these changes to values similar to those found in controls. Therefore, this study provides evidence, at the HI situ level, for cellular hyperplasia in heart failure. This supports the more general notion that adult cardiomyocytes may not be terminally differentiated, and that an increase in cell number could contribute to the increase in left ventricular mass observed with progression of disease.
AB - Based on positive identification of DNA replication and mitotic division in cardiomyocytes isolated from failing hearts, it has been proposed that adult ventricular cardiomyocytes can gain the capacity to proliferate with progression of heart failure. However, due to the lack of a reliable method to distinctly image individual cardiac cells within the myocardial syntitium, such a concept still remains largely controversial. In.the present study, we used laser confocal microscopy, to image cross-sections of intact myocardium stained with fluorescein-conjugated wheat germ agglutinin and propidium iodide. This approach allowed to clearly separate the profile of individual myocytcs within cardiac tissue sections. We found that in the left ventricles of dogs, subjected to tachycardiainduced cardiomyopathy, the number of cells was significantly increased in both longitudinal and transversal sections. Treatment with the angiotensin-converting enzyme inhibitor, enalapril, reversed these changes to values similar to those found in controls. Therefore, this study provides evidence, at the HI situ level, for cellular hyperplasia in heart failure. This supports the more general notion that adult cardiomyocytes may not be terminally differentiated, and that an increase in cell number could contribute to the increase in left ventricular mass observed with progression of disease.
KW - Angiotensinconverting enzyme
KW - Enalapril
KW - Fluorescein
KW - Heart failure
KW - Hyperplasia
KW - Laser confocal microscopy
KW - Morphometry
KW - Pacing
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UR - http://www.scopus.com/inward/citedby.url?scp=0033068602&partnerID=8YFLogxK
U2 - 10.3892/ijmm.3.2.153
DO - 10.3892/ijmm.3.2.153
M3 - Article
C2 - 9917522
AN - SCOPUS:0033068602
SN - 1107-3756
VL - 3
SP - 153
EP - 155
JO - International journal of molecular medicine
JF - International journal of molecular medicine
IS - 2
ER -