Increased levels of serum Wisteria floribunda agglutinin-positive Mac-2 binding protein in idiopathic pulmonary fibrosis

Masato Kono, Yutaro Nakamura, Yoshiyuki Oyama, Kazutaka Mori, Hironao Hozumi, Masato Karayama, Dai Hashimoto, Noriyuki Enomoto, Tomoyuki Fujisawa, Naoki Inui, Masaomi Yamada, Etsuko Hamada, Thomas V. Colby, Masato Maekawa, Takafumi Suda

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Abstract

Background Mac-2 binding protein (M2BP) is a cell-adhesive glycoprotein of the extracellular matrix secreted as a ligand of galectin-3 (Mac-2). Recently, a Wisteria floribunda agglutinin positive-M2BP (WFA+-M2BP) assay developed using a lectin-antibody sandwich immunoassay has shown promise as a new fibrotic marker in liver fibrosis to detect unique fibrosis-related glycoalteration. The aim of this study is to evaluate the utility of serum WFA+-M2BP levels in patients with idiopathic pulmonary fibrosis (IPF). Methods We measured serum WFA+-M2BP levels in 116 patients with IPF and 42 healthy volunteers. We examined the relationship between serum WFA+-M2BP levels and clinical parameters and further investigated the prognostic significance of serum WFA+-M2BP levels in patients with IPF. Results Serum WFA+-M2BP levels were significantly higher in patients with IPF than in healthy controls (1.09 ± 0.89 cutoff index [COI], 0.57 ± 0.24 COI, respectively; P <0.001). In patients with IPF, a significant positive correlation was found between serum WFA+-M2BP levels and age, KL-6, neutrophils in BAL, reticulation and honeycombing scores in HRCT, and fibrotic foci scores in pathological findings, and a significant negative correlation was found between serum WFA+-M2BP levels and FVC, %DLco and macrophages in BAL. Furthermore, patients with high serum WFA+-M2BP levels had a significantly worse prognosis than those with low levels (log-rank test, P = 0.0209), and a high serum WFA+-M2BP level was a significant prognostic factor in Cox proportional hazards regression analysis. Conclusions Our results suggest that the serum WFA+-M2BP level is a potential biomarker in patients with IPF.

Original languageEnglish (US)
Pages (from-to)46-52
Number of pages7
JournalRespiratory Medicine
Volume115
DOIs
StatePublished - Jun 1 2016

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Keywords

  • Biomarker
  • Idiopathic pulmonary fibrosis (IPF)
  • Mac-2 binding protein (M2BP)
  • Mac-2 binding protein glycosylation isomer (M2BPGi)
  • Wisteria floribunda agglutinin positive-M2BP (WFA-M2BP)

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Kono, M., Nakamura, Y., Oyama, Y., Mori, K., Hozumi, H., Karayama, M., Hashimoto, D., Enomoto, N., Fujisawa, T., Inui, N., Yamada, M., Hamada, E., Colby, T. V., Maekawa, M., & Suda, T. (2016). Increased levels of serum Wisteria floribunda agglutinin-positive Mac-2 binding protein in idiopathic pulmonary fibrosis. Respiratory Medicine, 115, 46-52. https://doi.org/10.1016/j.rmed.2016.04.013