TY - JOUR
T1 - Increased hepatic blood flow during enteral immune-enhancing diet gavage requires intact enterohepatic bile cycling
AU - Nagengast, Andrea K.
AU - Hurt, Ryan T.
AU - Downard, Cynthia D.
AU - Smith, Jason W.
AU - Garrison, R. Neal
AU - Matheson, Paul J.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/3
Y1 - 2014/3
N2 - Objectives: Total hepatic blood flow (HBF) via the hepatic artery and portal vein is highly dependent on gastrointestinal perfusion. During postprandial hyperemia, intestinal blood flow depends on nutrient composition, gastrointestinal location, and time. Immune-enhancing diets (IEDs) containing n-3 polyunsaturated fatty acids (PUFAs) selectively augment blood flow in the ileum at 60-120 min via a bile-dependent mechanism. My colleagues and I hypothesized that liver blood flow would be similarly affected by IEDs containing n-3 PUFAs. Methods: Mean arterial blood pressure, heart rate, and effective HBF (galactose clearance) were measured in anesthetized male Sprague-Dawley rats after gastric gavage of either a control diet (CD, Boost, Novartis) or an IED (Impact, Nestle Nutrition), with or without bile-duct ligation (BDL), and with or without supplemental bile (bovine, dried, unfractionated). Significance was assessed by 2-way ANOVA for repeated measures with the Tukey-Kramer honestly significant difference test. Results: Compared with baseline levels, a CD increased HBF (peak at 40 min *. P < 0.05) whereas an IED increased HBF in two distinct peaks at 40 min (*. P < 0.05) and 120 min (*. P < 0.05), but BDL prevented both the early (CD and IED, †. P < 0.05) and late peaks (IED, †. P < 0.05). Bile supplementation in the CD + BDL or IED + BDL groups restored neither the CD peak nor the early or late IED peaks. Conclusions: HBF during absorptive intestinal hyperemia is modulated by a mechanism that requires an intact enterohepatic circulation. The early peaks at 40 min (CD or IED) were prevented by BDL, even though fat absorption in the proximal gut occurs by bile-independent direct absorption. Bile supplementation with the diet (CD + BDL or IED + BDL) was insufficient to restore HBF hyperemia, which implies that a relationship exists between intestinal and hepatic blood flow that is not solely dependent on bile-mediated intestinal fat absorption and bile recirculation.
AB - Objectives: Total hepatic blood flow (HBF) via the hepatic artery and portal vein is highly dependent on gastrointestinal perfusion. During postprandial hyperemia, intestinal blood flow depends on nutrient composition, gastrointestinal location, and time. Immune-enhancing diets (IEDs) containing n-3 polyunsaturated fatty acids (PUFAs) selectively augment blood flow in the ileum at 60-120 min via a bile-dependent mechanism. My colleagues and I hypothesized that liver blood flow would be similarly affected by IEDs containing n-3 PUFAs. Methods: Mean arterial blood pressure, heart rate, and effective HBF (galactose clearance) were measured in anesthetized male Sprague-Dawley rats after gastric gavage of either a control diet (CD, Boost, Novartis) or an IED (Impact, Nestle Nutrition), with or without bile-duct ligation (BDL), and with or without supplemental bile (bovine, dried, unfractionated). Significance was assessed by 2-way ANOVA for repeated measures with the Tukey-Kramer honestly significant difference test. Results: Compared with baseline levels, a CD increased HBF (peak at 40 min *. P < 0.05) whereas an IED increased HBF in two distinct peaks at 40 min (*. P < 0.05) and 120 min (*. P < 0.05), but BDL prevented both the early (CD and IED, †. P < 0.05) and late peaks (IED, †. P < 0.05). Bile supplementation in the CD + BDL or IED + BDL groups restored neither the CD peak nor the early or late IED peaks. Conclusions: HBF during absorptive intestinal hyperemia is modulated by a mechanism that requires an intact enterohepatic circulation. The early peaks at 40 min (CD or IED) were prevented by BDL, even though fat absorption in the proximal gut occurs by bile-independent direct absorption. Bile supplementation with the diet (CD + BDL or IED + BDL) was insufficient to restore HBF hyperemia, which implies that a relationship exists between intestinal and hepatic blood flow that is not solely dependent on bile-mediated intestinal fat absorption and bile recirculation.
KW - Effective hepatic blood flow
KW - Enterohepatic bile transport
KW - Galactose clearance
KW - Immune-enhancing diet
KW - Intestinal absorptive hyperemia
KW - Intestinal lipid absorption
KW - Liver blood flow
KW - Postprandial hyperemia
KW - Sprague-Dawley rat
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U2 - 10.1016/j.nut.2013.08.006
DO - 10.1016/j.nut.2013.08.006
M3 - Article
C2 - 24355437
AN - SCOPUS:84894905446
SN - 0899-9007
VL - 30
SP - 313
EP - 318
JO - Nutrition
JF - Nutrition
IS - 3
ER -