TY - JOUR
T1 - Increased Expression of Hypoxia-inducible Factor-1α in Venous Stenosis of Arteriovenous Polytetrafluoroethylene Grafts in a Chronic Renal Insufficiency Porcine Model
AU - Misra, Sanjay
AU - Fu, Alex A.
AU - Puggioni, Alessandra
AU - Glockner, James F.
AU - Rajan, Dheeraj K.
AU - McKusick, Michael A.
AU - Bjarnason, Haraldur
AU - Mukhopadhyay, Debabrata
PY - 2008/2
Y1 - 2008/2
N2 - Purpose: To create a more clinically relevant model of hemodialysis graft failure in pigs by creating chronic renal insufficiency before polytetrafluoroethylene (PTFE) hemodialysis graft placement and to determine the expression of hypoxia-inducible factor-1α (HIF-1α) at the vein-to-graft anastomosis (VGA). Materials and Methods: Chronic renal insufficiency was created in 14 castrated juvenile male pigs with complete embolization of the left renal artery and the partial embolization of the right renal artery by infusing 150-250-μm polyvinyl acrylide spherical particles. The efficacy of the embolization was assessed by determining the amount of polyvinyl acrylide particles used per kidney, the weight of the kidneys at sacrifice, and kidney function (blood urea nitrogen [BUN] and creatinine levels). Twenty-eight days after embolization, PTFE grafts were placed from the carotid artery to the ipsilateral jugular vein and removed 3, 7, and 14 days after graft placement. Western blot for HIF-1α was performed in the VGA and control vessel. Results: The left kidney required two times the polyvinyl acrylide particles than did the right kidney (P < .05). The right kidney weighed nearly three times more than the left (P < .05). The BUN and creatinine levels at graft placement were significantly higher than those at baseline (P < .05). Four grafts were patent at day 3, four at day 7, and four at day 14. By day 7, the mean HIF-1α at the VGA had increased significantly when compared with that of control vessels (P < .05). Conclusions: A more clinically relevant porcine model of hemodialysis graft failure was created, and there was significantly increased expression of HIF-1α by day 7 at the VGA.
AB - Purpose: To create a more clinically relevant model of hemodialysis graft failure in pigs by creating chronic renal insufficiency before polytetrafluoroethylene (PTFE) hemodialysis graft placement and to determine the expression of hypoxia-inducible factor-1α (HIF-1α) at the vein-to-graft anastomosis (VGA). Materials and Methods: Chronic renal insufficiency was created in 14 castrated juvenile male pigs with complete embolization of the left renal artery and the partial embolization of the right renal artery by infusing 150-250-μm polyvinyl acrylide spherical particles. The efficacy of the embolization was assessed by determining the amount of polyvinyl acrylide particles used per kidney, the weight of the kidneys at sacrifice, and kidney function (blood urea nitrogen [BUN] and creatinine levels). Twenty-eight days after embolization, PTFE grafts were placed from the carotid artery to the ipsilateral jugular vein and removed 3, 7, and 14 days after graft placement. Western blot for HIF-1α was performed in the VGA and control vessel. Results: The left kidney required two times the polyvinyl acrylide particles than did the right kidney (P < .05). The right kidney weighed nearly three times more than the left (P < .05). The BUN and creatinine levels at graft placement were significantly higher than those at baseline (P < .05). Four grafts were patent at day 3, four at day 7, and four at day 14. By day 7, the mean HIF-1α at the VGA had increased significantly when compared with that of control vessels (P < .05). Conclusions: A more clinically relevant porcine model of hemodialysis graft failure was created, and there was significantly increased expression of HIF-1α by day 7 at the VGA.
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U2 - 10.1016/j.jvir.2007.10.029
DO - 10.1016/j.jvir.2007.10.029
M3 - Article
C2 - 18341959
AN - SCOPUS:38349168182
SN - 1051-0443
VL - 19
SP - 260
EP - 265
JO - Journal of Vascular and Interventional Radiology
JF - Journal of Vascular and Interventional Radiology
IS - 2
ER -