Increased Expression of Hypoxia-inducible Factor-1α in Venous Stenosis of Arteriovenous Polytetrafluoroethylene Grafts in a Chronic Renal Insufficiency Porcine Model

Sanjay Misra, Alex A. Fu, Alessandra Puggioni, James Glockner, Dheeraj K. Rajan, Michael A. McKusick, Haraldur Bjarnason, Debabrata Mukhopadhyay

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Purpose: To create a more clinically relevant model of hemodialysis graft failure in pigs by creating chronic renal insufficiency before polytetrafluoroethylene (PTFE) hemodialysis graft placement and to determine the expression of hypoxia-inducible factor-1α (HIF-1α) at the vein-to-graft anastomosis (VGA). Materials and Methods: Chronic renal insufficiency was created in 14 castrated juvenile male pigs with complete embolization of the left renal artery and the partial embolization of the right renal artery by infusing 150-250-μm polyvinyl acrylide spherical particles. The efficacy of the embolization was assessed by determining the amount of polyvinyl acrylide particles used per kidney, the weight of the kidneys at sacrifice, and kidney function (blood urea nitrogen [BUN] and creatinine levels). Twenty-eight days after embolization, PTFE grafts were placed from the carotid artery to the ipsilateral jugular vein and removed 3, 7, and 14 days after graft placement. Western blot for HIF-1α was performed in the VGA and control vessel. Results: The left kidney required two times the polyvinyl acrylide particles than did the right kidney (P < .05). The right kidney weighed nearly three times more than the left (P < .05). The BUN and creatinine levels at graft placement were significantly higher than those at baseline (P < .05). Four grafts were patent at day 3, four at day 7, and four at day 14. By day 7, the mean HIF-1α at the VGA had increased significantly when compared with that of control vessels (P < .05). Conclusions: A more clinically relevant porcine model of hemodialysis graft failure was created, and there was significantly increased expression of HIF-1α by day 7 at the VGA.

Original languageEnglish (US)
Pages (from-to)260-265
Number of pages6
JournalJournal of Vascular and Interventional Radiology
Volume19
Issue number2
DOIs
StatePublished - Feb 2008

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Hypoxia-Inducible Factor 1
Polytetrafluoroethylene
Chronic Renal Insufficiency
Pathologic Constriction
Swine
Transplants
Polyvinyls
Kidney
Veins
Renal Dialysis
Blood Urea Nitrogen
Renal Artery
Creatinine
Jugular Veins
Carotid Arteries
Western Blotting

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Increased Expression of Hypoxia-inducible Factor-1α in Venous Stenosis of Arteriovenous Polytetrafluoroethylene Grafts in a Chronic Renal Insufficiency Porcine Model. / Misra, Sanjay; Fu, Alex A.; Puggioni, Alessandra; Glockner, James; Rajan, Dheeraj K.; McKusick, Michael A.; Bjarnason, Haraldur; Mukhopadhyay, Debabrata.

In: Journal of Vascular and Interventional Radiology, Vol. 19, No. 2, 02.2008, p. 260-265.

Research output: Contribution to journalArticle

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abstract = "Purpose: To create a more clinically relevant model of hemodialysis graft failure in pigs by creating chronic renal insufficiency before polytetrafluoroethylene (PTFE) hemodialysis graft placement and to determine the expression of hypoxia-inducible factor-1α (HIF-1α) at the vein-to-graft anastomosis (VGA). Materials and Methods: Chronic renal insufficiency was created in 14 castrated juvenile male pigs with complete embolization of the left renal artery and the partial embolization of the right renal artery by infusing 150-250-μm polyvinyl acrylide spherical particles. The efficacy of the embolization was assessed by determining the amount of polyvinyl acrylide particles used per kidney, the weight of the kidneys at sacrifice, and kidney function (blood urea nitrogen [BUN] and creatinine levels). Twenty-eight days after embolization, PTFE grafts were placed from the carotid artery to the ipsilateral jugular vein and removed 3, 7, and 14 days after graft placement. Western blot for HIF-1α was performed in the VGA and control vessel. Results: The left kidney required two times the polyvinyl acrylide particles than did the right kidney (P < .05). The right kidney weighed nearly three times more than the left (P < .05). The BUN and creatinine levels at graft placement were significantly higher than those at baseline (P < .05). Four grafts were patent at day 3, four at day 7, and four at day 14. By day 7, the mean HIF-1α at the VGA had increased significantly when compared with that of control vessels (P < .05). Conclusions: A more clinically relevant porcine model of hemodialysis graft failure was created, and there was significantly increased expression of HIF-1α by day 7 at the VGA.",
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T1 - Increased Expression of Hypoxia-inducible Factor-1α in Venous Stenosis of Arteriovenous Polytetrafluoroethylene Grafts in a Chronic Renal Insufficiency Porcine Model

AU - Misra, Sanjay

AU - Fu, Alex A.

AU - Puggioni, Alessandra

AU - Glockner, James

AU - Rajan, Dheeraj K.

AU - McKusick, Michael A.

AU - Bjarnason, Haraldur

AU - Mukhopadhyay, Debabrata

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N2 - Purpose: To create a more clinically relevant model of hemodialysis graft failure in pigs by creating chronic renal insufficiency before polytetrafluoroethylene (PTFE) hemodialysis graft placement and to determine the expression of hypoxia-inducible factor-1α (HIF-1α) at the vein-to-graft anastomosis (VGA). Materials and Methods: Chronic renal insufficiency was created in 14 castrated juvenile male pigs with complete embolization of the left renal artery and the partial embolization of the right renal artery by infusing 150-250-μm polyvinyl acrylide spherical particles. The efficacy of the embolization was assessed by determining the amount of polyvinyl acrylide particles used per kidney, the weight of the kidneys at sacrifice, and kidney function (blood urea nitrogen [BUN] and creatinine levels). Twenty-eight days after embolization, PTFE grafts were placed from the carotid artery to the ipsilateral jugular vein and removed 3, 7, and 14 days after graft placement. Western blot for HIF-1α was performed in the VGA and control vessel. Results: The left kidney required two times the polyvinyl acrylide particles than did the right kidney (P < .05). The right kidney weighed nearly three times more than the left (P < .05). The BUN and creatinine levels at graft placement were significantly higher than those at baseline (P < .05). Four grafts were patent at day 3, four at day 7, and four at day 14. By day 7, the mean HIF-1α at the VGA had increased significantly when compared with that of control vessels (P < .05). Conclusions: A more clinically relevant porcine model of hemodialysis graft failure was created, and there was significantly increased expression of HIF-1α by day 7 at the VGA.

AB - Purpose: To create a more clinically relevant model of hemodialysis graft failure in pigs by creating chronic renal insufficiency before polytetrafluoroethylene (PTFE) hemodialysis graft placement and to determine the expression of hypoxia-inducible factor-1α (HIF-1α) at the vein-to-graft anastomosis (VGA). Materials and Methods: Chronic renal insufficiency was created in 14 castrated juvenile male pigs with complete embolization of the left renal artery and the partial embolization of the right renal artery by infusing 150-250-μm polyvinyl acrylide spherical particles. The efficacy of the embolization was assessed by determining the amount of polyvinyl acrylide particles used per kidney, the weight of the kidneys at sacrifice, and kidney function (blood urea nitrogen [BUN] and creatinine levels). Twenty-eight days after embolization, PTFE grafts were placed from the carotid artery to the ipsilateral jugular vein and removed 3, 7, and 14 days after graft placement. Western blot for HIF-1α was performed in the VGA and control vessel. Results: The left kidney required two times the polyvinyl acrylide particles than did the right kidney (P < .05). The right kidney weighed nearly three times more than the left (P < .05). The BUN and creatinine levels at graft placement were significantly higher than those at baseline (P < .05). Four grafts were patent at day 3, four at day 7, and four at day 14. By day 7, the mean HIF-1α at the VGA had increased significantly when compared with that of control vessels (P < .05). Conclusions: A more clinically relevant porcine model of hemodialysis graft failure was created, and there was significantly increased expression of HIF-1α by day 7 at the VGA.

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