TY - JOUR
T1 - Incorporation of simian virus 5 fusion protein into murine leukemia virus particles and its effect on the co-incorporation of retroviral envelope glycoproteins
AU - Hatziioannou, Theodora
AU - Russell, Stephen J.
AU - Cosset, François Loïc
N1 - Funding Information:
We are grateful to Dr. R. Lamb for his generous gifts of plasmids and antibodies. This work was supported by Agence Nationale pour la Recherche contre le SIDA (ANRS), Association pour la Recherche contre le Cancer (ARC). The European Community, and Institut National delaSantéEtdelaRechercheMédicale(INSERM).T.H.wassupported by a fellowship from the European Community and by the ARC.
PY - 2000/2/1
Y1 - 2000/2/1
N2 - We describe the generation of murine leukemia virus (MLV) virus particles carrying the paramyxovirus fusion protein F from simian virus 5 (SV5-F). This glycoprotein was expressed in cells providing Moloney MLV (MoMLV) Gag and Pol proteins and a lacZ retroviral vector. SV5-F was correctly expressed, processed, and efficiently incorporated into retroviral particles. SV5-F-bearing retroviruses were not infectious although a weak binding to primate and rodent cells could be detected and SV5-F could mediate cell to cell fusion. We then co-expressed the SV5-F glycoprotein in retroviral particles with chimeric and wild-type MoMLV envelope glycoproteins. Our results show that F strongly inhibited infection via the retroviral envelopes although the mechanism of inhibition was different depending on the retroviral envelope used. (C) 2000 Academic Press.
AB - We describe the generation of murine leukemia virus (MLV) virus particles carrying the paramyxovirus fusion protein F from simian virus 5 (SV5-F). This glycoprotein was expressed in cells providing Moloney MLV (MoMLV) Gag and Pol proteins and a lacZ retroviral vector. SV5-F was correctly expressed, processed, and efficiently incorporated into retroviral particles. SV5-F-bearing retroviruses were not infectious although a weak binding to primate and rodent cells could be detected and SV5-F could mediate cell to cell fusion. We then co-expressed the SV5-F glycoprotein in retroviral particles with chimeric and wild-type MoMLV envelope glycoproteins. Our results show that F strongly inhibited infection via the retroviral envelopes although the mechanism of inhibition was different depending on the retroviral envelope used. (C) 2000 Academic Press.
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U2 - 10.1006/viro.1999.0091
DO - 10.1006/viro.1999.0091
M3 - Article
C2 - 10648182
AN - SCOPUS:0034141367
SN - 0042-6822
VL - 267
SP - 49
EP - 57
JO - Virology
JF - Virology
IS - 1
ER -