Inclusion body myositis: Laser microdissection reveals differential Up-regulation of IFN-γ signaling cascade in attacked versus nonattacked myofibers

Jana Ivanidze, Reinhard Hoffmann, Hanns Lochmller, Andrew G. Engel, Reinhard Hohlfeld, Klaus Dornmair

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Sporadic inclusion body myositis (IBM) is a muscle disease with two separate pathogenic components, degeneration and inflammation. Typically, nonnecrotic myofibers are focally surrounded and invaded by CD8 + T cells and macrophages. Both attacked and nonattacked myofibers express high levels of human leukocyte antigen class I (HLA-I) molecules, a prerequisite for antigen presentation to CD8 + T cells. However, only a subgroup of HLA-I + myofibers is attacked by immune cells. By using IHC, we classified myofibers from five patients with sporadic IBM as attacked (A IBM) or nonattacked (N IBM) and isolated the intracellular contents of myofibers separately by laser microdissection. For comparison, we isolated myofibers from control persons (H CTRL). The samples were analyzed by microarray hybridization and quantitative PCR. HLA-I up-regulation was observed in A IBM and N IBM, whereas H CTRL were negative for HLA-I. In contrast, the inducible chain of the interferon (IFN) γ receptor (IFNGR2) and several IFN-γinduced genes were up-regulated in A IBM compared with N IBM and H CTRL fibers. Confocal microscopy confirmed segmental IFNGR2 up-regulation on the membranes of A IBM, which positively correlated with the number of adjacent CD8 + T cells. Thus, the differential up-regulation of the IFN-γ signaling cascade observed in the attacked fibers is related to local inflammation, whereas the ubiquitous HLA-I expression on IBM muscle fibers does not require IFNGR expression.

Original languageEnglish (US)
Pages (from-to)1347-1359
Number of pages13
JournalAmerican Journal of Pathology
Volume179
Issue number3
DOIs
StatePublished - Sep 2011

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Inclusion body myositis: Laser microdissection reveals differential Up-regulation of IFN-γ signaling cascade in attacked versus nonattacked myofibers'. Together they form a unique fingerprint.

Cite this