Incidence of TCR and TCL1 gene translocations and isochromosome 7q in peripheral T-cell lymphomas using fluorescence in situ hybridization

Andrew L Feldman, Mark Law, Karen L. Grogg, Erik C Thorland, Stephanie Fink, Paul J. Kurtin, William R. Macon, Ellen McPhail, Ahmet Dogan

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Translocations involving the T-cell receptor (TCR) and TCL1 genes occur in T-cell precursor lymphoblastic leukemia/lymphoma and prolymphocytic leukemia; isochromosome 7q has been associated with hepatosplenic T-cell lymphoma. However, the incidence of these abnormalities in peripheral T-cell lymphomas (PTCLs) as a whole has not been well defined. We studied genetic abnormalities in 124 PTCLs seen at the Mayo Clinic, Rochester, MN, between 1987 and 2007. Tissue microarrays were screened using 2-color break-apart fluorescence in situ hybridization probes flanking the TCRα (TCRA, 14q11), TCRβ (TCRB, 7q35), and TCRaγ (TCRG, 7p15) genes and the TCL1 gene (14q32). Isochromosome 7q was analyzed by using a 2-color probe to 7p and 7q32.1. Translocations involved TCRA in 3 (2.9%) of 102 cases and TCRB in 1 (1%) of 88. Isochromosome 7q was detected in 2 cases of extranodal NK/T-cell lymphoma, nasal type, and 2 cases of anaplastic lymphoma kinase-negative anaplastic large cell lymphoma. One of the latter cases also had a translocation of TCRA, and further studies confirmed a novel t(5;14) translocation.

Original languageEnglish (US)
Pages (from-to)178-185
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume130
Issue number2
DOIs
StatePublished - Aug 2008

Fingerprint

Isochromosomes
Peripheral T-Cell Lymphoma
T-Cell Receptor Genes
Fluorescence In Situ Hybridization
T-Cell Antigen Receptor
Incidence
Extranodal NK-T-Cell Lymphoma
Prolymphocytic Leukemia
Color
Anaplastic Large-Cell Lymphoma
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
T-Cell Lymphoma
Genes

Keywords

  • Cytogenetics
  • Extranodal NK/T-cell lymphoma
  • Fluorescence in situ hybridization
  • Isochromosome 7q
  • Peripheral T-cell lymphoma
  • T-cell receptor
  • TCL1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Incidence of TCR and TCL1 gene translocations and isochromosome 7q in peripheral T-cell lymphomas using fluorescence in situ hybridization. / Feldman, Andrew L; Law, Mark; Grogg, Karen L.; Thorland, Erik C; Fink, Stephanie; Kurtin, Paul J.; Macon, William R.; McPhail, Ellen; Dogan, Ahmet.

In: American Journal of Clinical Pathology, Vol. 130, No. 2, 08.2008, p. 178-185.

Research output: Contribution to journalArticle

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