Incidence of TCR and TCL1 gene translocations and isochromosome 7q in peripheral T-cell lymphomas using fluorescence in situ hybridization

Andrew L. Feldman, Mark Law, Karen L. Grogg, Erik C. Thorland, Stephanie Fink, Paul J. Kurtin, William R. Macon, Ellen D. Remstein, Ahmet Dogan

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Translocations involving the T-cell receptor (TCR) and TCL1 genes occur in T-cell precursor lymphoblastic leukemia/lymphoma and prolymphocytic leukemia; isochromosome 7q has been associated with hepatosplenic T-cell lymphoma. However, the incidence of these abnormalities in peripheral T-cell lymphomas (PTCLs) as a whole has not been well defined. We studied genetic abnormalities in 124 PTCLs seen at the Mayo Clinic, Rochester, MN, between 1987 and 2007. Tissue microarrays were screened using 2-color break-apart fluorescence in situ hybridization probes flanking the TCRα (TCRA, 14q11), TCRβ (TCRB, 7q35), and TCRaγ (TCRG, 7p15) genes and the TCL1 gene (14q32). Isochromosome 7q was analyzed by using a 2-color probe to 7p and 7q32.1. Translocations involved TCRA in 3 (2.9%) of 102 cases and TCRB in 1 (1%) of 88. Isochromosome 7q was detected in 2 cases of extranodal NK/T-cell lymphoma, nasal type, and 2 cases of anaplastic lymphoma kinase-negative anaplastic large cell lymphoma. One of the latter cases also had a translocation of TCRA, and further studies confirmed a novel t(5;14) translocation.

Original languageEnglish (US)
Pages (from-to)178-185
Number of pages8
JournalAmerican journal of clinical pathology
Volume130
Issue number2
DOIs
StatePublished - Aug 2008

Keywords

  • Cytogenetics
  • Extranodal NK/T-cell lymphoma
  • Fluorescence in situ hybridization
  • Isochromosome 7q
  • Peripheral T-cell lymphoma
  • T-cell receptor
  • TCL1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Incidence of TCR and TCL1 gene translocations and isochromosome 7q in peripheral T-cell lymphomas using fluorescence in situ hybridization'. Together they form a unique fingerprint.

Cite this