We electrophoresed serum samples on Mylar-backed cellulose acetate membranes and stained for glycoproteins with the periodic acid-Schiff reagent. The samples were from untreated adenocarcinoma patients, adenocarcinoma patients receiving chemotherapy, and patients with other malignancies, and also from patients with benign proliferative diseases, inflammatory diseases, and other nonmalignant conditions. Forty-five per cent of the sera from untreated adenocarcinoma patients and 80% of those from adenocarcinoma patients with progressive systemic disease exhibited a splitting of the α2-glycoproteins into a fast and slow band. Such a pattern was seen in only 4% of the non-adenocarcinoma cancer patients and 4% of the control group. Serial studies indicated that electrophoretic patterns of α2-glycoproteins change with clinical status. Non-cancer patients with high concentrations of acute-phase proteins in their serum did not exhibit two α2-glycoprotein bands. Further characterization of serum proteins from the fast α2 region suggest that α1-acid glycoprotein and haptoglobin beta chains are the principal components staining with periodic acid-Schiff reagent. These components are markedly less apparent in, or are absent from, the fast α2 region of normal sera.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Jan 1 1980|
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical