In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis

Sara Zanivan, Alexander Meves, Kristina Behrendt, Erwin M. Schoof, Lisa J. Neilson, Jürgen Cox, Hao R. Tang, Gabriela Kalna, Janine H. van Ree, Jan Van Deursen, Carol S. Trempus, Laura M. Machesky, Rune Linding, Sara A. Wickström, Reinhard Fässler, Matthias Mann

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Cancer progresses through distinct stages, and mouse models recapitulating traits of this progression are frequently used to explore genetic, morphological, and pharmacological aspects of tumor development. To complement genomic investigations of this process, we here quantify phosphoproteomic changes in skin cancer development using the SILAC mouse technology coupled to high-resolution mass spectrometry. We distill protein expression signatures from our data that distinguish between skin cancer stages. A distinct phosphoproteome of the two stages of cancer progression is identified that correlates with perturbed cell growth and implicates cell adhesion as a major driver of malignancy. Importantly, integrated analysis of phosphoproteomic data and prediction of kinase activity revealed PAK4-PKC/SRC network to be highly deregulated in SCC but not in papilloma. This detailed molecular picture, both at the proteome and phosphoproteome level, will prove useful for the study of mechanisms of tumor progression.

Original languageEnglish (US)
Pages (from-to)552-566
Number of pages15
JournalCell Reports
Volume3
Issue number2
DOIs
StatePublished - 2013

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Proteomics
Tumors
Skin
Carcinogenesis
Cell adhesion
Cell growth
Proteome
Mass spectrometry
Skin Neoplasms
Neoplasms
Phosphotransferases
Papilloma
Proteins
Cell Adhesion
Mass Spectrometry
Pharmacology
Technology
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis. / Zanivan, Sara; Meves, Alexander; Behrendt, Kristina; Schoof, Erwin M.; Neilson, Lisa J.; Cox, Jürgen; Tang, Hao R.; Kalna, Gabriela; van Ree, Janine H.; Van Deursen, Jan; Trempus, Carol S.; Machesky, Laura M.; Linding, Rune; Wickström, Sara A.; Fässler, Reinhard; Mann, Matthias.

In: Cell Reports, Vol. 3, No. 2, 2013, p. 552-566.

Research output: Contribution to journalArticle

Zanivan, S, Meves, A, Behrendt, K, Schoof, EM, Neilson, LJ, Cox, J, Tang, HR, Kalna, G, van Ree, JH, Van Deursen, J, Trempus, CS, Machesky, LM, Linding, R, Wickström, SA, Fässler, R & Mann, M 2013, 'In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis', Cell Reports, vol. 3, no. 2, pp. 552-566. https://doi.org/10.1016/j.celrep.2013.01.003
Zanivan, Sara ; Meves, Alexander ; Behrendt, Kristina ; Schoof, Erwin M. ; Neilson, Lisa J. ; Cox, Jürgen ; Tang, Hao R. ; Kalna, Gabriela ; van Ree, Janine H. ; Van Deursen, Jan ; Trempus, Carol S. ; Machesky, Laura M. ; Linding, Rune ; Wickström, Sara A. ; Fässler, Reinhard ; Mann, Matthias. / In Vivo SILAC-Based Proteomics Reveals Phosphoproteome Changes during Mouse Skin Carcinogenesis. In: Cell Reports. 2013 ; Vol. 3, No. 2. pp. 552-566.
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